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| Status |
Public on Apr 19, 2016 |
| Title |
H3K27me3 ChIP-sequencing on LSK (Lin-Sca-1+c-kit+) cells isolated from control, MxCre Jak2VF/+ and MxCre Jak2VF/+ EZH2-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Inactivating EZH2 mutations have been associated with myelofibrosis (MF). Moreover, EZH2 mutations co-exist with the JAK2V617F mutation in a significant cases of MF. To determine the effects of concomitant loss of EZH2 and JAK2V617F mutation in hematopoiesis, we generated Ezh2-deficient Jak2V617F-expressing mice. To identify the Ezh2 target genes that are regulated by H3K27me3 in MF, we performed H3K27me3 ChIP-sequencing in sorted LSK cells from control, MxCre;Jak2VF/+ and MxCre;Jak2VF/+ EZH2-/- mice.
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| Overall design |
1500μg pI-pC was administrated to control, MxCre Jak2VF/+ and MxCre Jak2VF/+ EZH2-/- at 4 weeks after birth in five times (300μg each time). Bone marrow cells were harvested from the mice at 12 weeks after pI-pC injections.ChIP-seq experiments were performed for LSK cells sorted from BM cells.
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| Contributor(s) |
Yang Y, Mohi G |
| Citation(s) |
27081096 |
| Submission date |
Mar 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Golam Mohi |
| E-mail(s) |
mohim@upstate.edu
|
| Organization name |
SUNY Upstate Medical University
|
| Department |
Pharmacology
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| Street address |
766 Irving Avenue
|
| City |
Syracuse |
| State/province |
New York |
| ZIP/Postal code |
13210 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE79472 |
Loss of Ezh2 cooperates with Jak2V617F in the development of myelofibrosis in a mouse model of myeloproliferative neoplasm |
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| Relations |
| BioProject |
PRJNA315452 |
| SRA |
SRP071899 |
