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Series GSE79319 Query DataSets for GSE79319
Status Public on Apr 19, 2016
Title H3K27me3 ChIP-sequencing on LSK (Lin-Sca-1+c-kit+) cells isolated from control, MxCre Jak2VF/+ and MxCre Jak2VF/+ EZH2-/- mice
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Inactivating EZH2 mutations have been associated with myelofibrosis (MF). Moreover, EZH2 mutations co-exist with the JAK2V617F mutation in a significant cases of MF. To determine the effects of concomitant loss of EZH2 and JAK2V617F mutation in hematopoiesis, we generated Ezh2-deficient Jak2V617F-expressing mice. To identify the Ezh2 target genes that are regulated by H3K27me3 in MF, we performed H3K27me3 ChIP-sequencing in sorted LSK cells from control, MxCre;Jak2VF/+ and MxCre;Jak2VF/+ EZH2-/- mice.
Overall design 1500μg pI-pC was administrated to control, MxCre Jak2VF/+ and MxCre Jak2VF/+ EZH2-/- at 4 weeks after birth in five times (300μg each time). Bone marrow cells were harvested from the mice at 12 weeks after pI-pC injections.ChIP-seq experiments were performed for LSK cells sorted from BM cells.
Contributor(s) Yang Y, Mohi G
Citation(s) 27081096
Submission date Mar 16, 2016
Last update date May 15, 2019
Contact name Golam Mohi
Organization name SUNY Upstate Medical University
Department Pharmacology
Street address 766 Irving Avenue
City Syracuse
State/province New York
ZIP/Postal code 13210
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (3)
GSM2091489 WT_H3K27me3 ChIP-seq
GSM2091490 MxCre Jak2VF/+_H3K27me3 ChIP-seq
GSM2091491 MxCre Jak2VF/+ EZH2-/-_H3K27me3 ChIP-seq
This SubSeries is part of SuperSeries:
GSE79472 Loss of Ezh2 cooperates with Jak2V617F in the development of myelofibrosis in a mouse model of myeloproliferative neoplasm
BioProject PRJNA315452
SRA SRP071899

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Supplementary file Size Download File type/resource
GSE79319_RAW.tar 142.6 Mb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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