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Series GSE79296 Query DataSets for GSE79296
Status Public on Dec 07, 2016
Title m6A controls neurogenesis and sex determination in Drosophila via its nuclear reader protein YT521-B [MeRIP-Seq, S2R+ cells]
Organism Drosophila melanogaster
Experiment type Other
Summary N6-methyladenosine RNA (m6A) is the most abundant internal mRNA modification in mammals. While its role in the regulation of posttranscriptional gene expression is beginning to be unveiled, its function during development of complex organisms is poorly understood. Here, we identify Spenito as a novel member of the methyltransferase complex and show that m6A in Drosophila is necessary for proper synaptic growth, and in regulation of early steps of pre-mRNA splicing. Splicing of Sex-lethal and of its downstream targets are defective in animals lacking m6A, revealing also important roles in sex determination and dosage compensation. Finally, we implicate the nuclear m6A reader protein, YT521-B, as a crucial effector of m6A modifications in vivo. Altogether, our work provides important novel insights into m6A biology through identification and characterization of both m6A-writing and -reading proteins in Drosophila and their effects on splicing, neurogenesis and sex-determination within the context of the whole animal.
Overall design meRIP-Seq in S2R+ cells (2 conditions, duplicates)
Contributor(s) Lence T, Akhtar J, Bayer M, Schmid K, Spindler L, Ho CH, Kreim N, Andrade-Navarro MA, Poeck B, Helm M, Roignant J
Citation(s) 27919077
Submission date Mar 16, 2016
Last update date May 15, 2019
Contact name Jean-Yves Roignant
Organization name Institute of molecular Biology
Lab Roignant
Street address Ackermannweg 4
City Mainz
ZIP/Postal code 55128
Country Germany
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (4)
GSM2091061 Input1
GSM2091062 Input2
GSM2091063 MeRIP1
This SubSeries is part of SuperSeries:
GSE79297 m6A controls neurogenesis and sex determination in Drosophila via its nuclear reader protein YT521-B
BioProject PRJNA315420
SRA SRP071866

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Supplementary file Size Download File type/resource
GSE79296_RAW.tar 39.4 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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