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Series GSE79134 Query DataSets for GSE79134
Status Public on Feb 10, 2017
Title Optimized Approaches for Generation of Integration-free iPSCs from Human Urine-derived Cells with Small Molecules and Autologous Feeder
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We generated iPSCs from human urine cells (hUCs) with the aid of small molecules and autologous hUC feeders. A compound cocktail including Cyclic Pifithrin-a, a p53 inhibitor and other compounds known for benefiting reprogramming like A-83-01, CHIR99021, Thiazovivin, NaB and PD0325901 was used to aid hUC reprogramming (Plan B). Aided by this cocktail, we achieved significantly improved efficiency (170 folds more) for hUC reprogramming and iPSC generation. In addition, to enable iPSC generation in some cases that massive cell death occurred during delivering reprogramming factors, we replaced Matrigel with autologous hUCs as feeder for reprogramming and iPSC generation (Plan C). Replacing Matrigel with autologous feeder not only enhanced reprograming, but also avoided concern using animal components for human iPSC generation. These were efficient approaches to enable iPSC generation from hUCs that were otherwise difficult for reprogramming, which would be valuable for banking patient’s specific iPSCs.
Overall design We compared the transcriptome of hESCs, hiPSC, hUC by RNA-Seq. hiPSCs were generated from Plan B and Plan C. Plan B: RM at day 0~1, RM+5M at day 2~9, mTeSR1+6M at day10~17. UCs were seeded on Matrigel. Plan C: Medium was the same with Plan B, but seeded on autologous UCs as feeders before nucleofection.
Contributor(s) Li D, Chen Q, Yang Z, Hutchins A, Pan G, Du J
Citation(s) 27132887
Submission date Mar 11, 2016
Last update date May 15, 2019
Contact name qianyu chen
Phone +8602032015236
Organization name Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
Lab Guangjin Pan
Street address 190 Kai Yuan Avenue, Science Park
City Guangzhou
ZIP/Postal code 510530
Country China
Platforms (2)
GPL15520 Illumina MiSeq (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (5)
GSM2086457 hUC-HM1
GSM2086458 HM1-hiPSC-Plan B
GSM2086459 HF1-hiPSC-Plan B
BioProject PRJNA314996
SRA SRP071671

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79134_RAW.tar 1.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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