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Series GSE79128 Query DataSets for GSE79128
Status Public on May 20, 2016
Title AR and ERG ChIP-seq in presence or absence of PRMT5
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Replicates of ChIP seq data using AR and ERG antibodies from VCaP cell lines harboring inducible shRNA constructs for PRMT5 (3 independent, sh1, sh2, sh3) and 1 non targeting control (NTC), all sample stimulated with ligand, either DHT or R1881
Overall design AR ChIPseq in VCaP cells, 3 replicates each of DHT stimulated cells with one of 4 inducible shRNA constructs, sh1, sh2, sh3 targeting PRMT5 or NTC non targeting controls. Furthermore 2 AR ChIPseq in the same system stimulated with R1881 for targeting constructs sh1, sh2 and NTC. ERG ChIPseq 2 replicates DHT stimulated, each of sh1, sh2, sh3 constructs. Cells where induced (doxycyclin) for shRNA expression on day0, hormone starved on day3 and harvested on day5.
Contributor(s) Westerling T, Mounir Z, Pagliarini R, Brown M
Citation(s) 27183006
Submission date Mar 11, 2016
Last update date May 15, 2019
Contact name Thomas Westerling
Organization name Dana Farber Cancer institute
Department Medical Oncology
Lab Myles Brown
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (26)
GSM2086291 NTC DHT 1 AR
GSM2086292 sh1 DHT 1 AR
GSM2086293 sh2 DHT 1 AR
BioProject PRJNA314989
SRA SRP071668

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79128_MatrixData_allExamindedPeaks_NormalizedReadCount_MounirEtAl_2016.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79128_RAW.tar 2.8 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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