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Series GSE79108 Query DataSets for GSE79108
Status Public on May 23, 2017
Title Identification of preoptic sleep-promoting neurons based on projection target
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The preoptic area (POA) of the hypothalamus is known to be crucial for sleep generation, but the spatial intermingling of sleep- and wake-promoting neurons makes it difficult to dissect the sleep control circuit. Here we identified a population of POA sleep-promoting neurons based on their projection target. Using a lentivirus for retrograde labeling with channelrhodopsin-2 (ChR2) followed by optogenetic manipulation and recording, we found that the POA GABAergic neurons projecting to the tuberomammillary nucleus (TMN) are both sleep active and sleep promoting. Cell type- and projection-specific rabies tracing revealed the presynaptic inputs to these neurons, including an amygdala GABAergic input that promotes wakefulness. Using single-cell RNA-seq, we identified several molecular markers for these neurons, and optogenetic activation of the POA neurons labeled by these markers confirmed their sleep-promoting effects. Together, these findings define a group of sleep-promoting neurons functionally, anatomically, and genetically.
Overall design Single-cell RNA-Seq of retrogradely-labeled POA neurons projecting to the tuberomammillary nucleus (TMN).
Contributor(s) Tasic B, Nguyen T, Chung S, Dan Y
Citation(s) 28514446
Submission date Mar 11, 2016
Last update date May 15, 2019
Contact name Thuc Nghi Nguyen
Organization name Allen Institute for Brain Science
Street address 615 Westlake Ave N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (84)
GSM2085880 GAD_posterior_cell_1
GSM2085881 GAD_posterior_cell_2
GSM2085882 GAD_posterior_cell_3
BioProject PRJNA314957
SRA SRP071658

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Supplementary file Size Download File type/resource
GSE79108_ERCC_tdTomato_YFP_RPKM.csv.gz 15.0 Kb (ftp)(http) CSV
GSE79108_ERCC_tdTomato_YFP_counts.csv.gz 5.7 Kb (ftp)(http) CSV
GSE79108_ERCC_tdTomato_counts.csv.gz 5.5 Kb (ftp)(http) CSV
GSE79108_RefSeq_RPKM.csv.gz 1.7 Mb (ftp)(http) CSV
GSE79108_RefSeq_TPM.csv.gz 1.8 Mb (ftp)(http) CSV
GSE79108_RefSeq_counts.csv.gz 1.7 Mb (ftp)(http) CSV
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