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Series GSE79095 Query DataSets for GSE79095
Status Public on Jun 01, 2016
Title Alternative splicing regulation by homologous Muscleblind proteins
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We provide data showing alternative splicing regulation by Muscleblind proteins in MEFs. MEFs lacking functional Muscleblind (DKO MEFs) were stably reconstituted with Muscleblind proteins from Homo sapiens, Ciona intestinalis, Drosophila melanogaster, Caenorhabditis elegans or Trichoplax adhaerens and splicing regulation was explored using RNA-seq analysis followed by MISO (Mixture of Isoforms).
Overall design Alternative splicing was accessed using RNA-sequencing data from five DKO MEF lines reconstituted with different GFP-tagged Muscleblind homologs or GFP alone and compared to RNA-seq data from three WT MEF lines and three control DKO MEFs (no Muscleblind reconstitution). A total of 12 samples were used for high-throughput sequencing.
Contributor(s) Wang ET, Oddo JC, Saxena T
Citation(s) 27557707
NIH grant(s)
Grant ID Grant title Affiliation Name
DP5 OD017865 Post-transcriptional regulation of gene expression in neuromuscular disease: Ppst-transcriptional regulation of gene expression in neuromuscular disease MASSACHUSETTS INSTITUTE OF TECHNOLOGY ERIC T WANG
Submission date Mar 11, 2016
Last update date May 15, 2019
Contact name Eric T Wang
Organization name University of Florida
Street address 2033 Mowry Rd
City Gainesville
State/province FL
ZIP/Postal code 32610
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM2085307 WT Clone 1
GSM2085308 WT Clone 2
GSM2085309 WT Clone 3
BioProject PRJNA314940
SRA SRP071635

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79095_monotonicTotal.txt.gz 437.6 Kb (ftp)(http) TXT
GSE79095_nonUTRevents_819_consolidated.txt.gz 1.2 Mb (ftp)(http) TXT
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Raw data are available in SRA

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