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Series GSE78711 Query DataSets for GSE78711
Status Public on Mar 04, 2016
Title Zika Virus Targets Human Cortical Neural Precursors and Attenuates Their Growth
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly, is an urgent global health concern. The direct target cells of ZIKV in developing human fetuses are not clear. Here we show that ZIKV serially passaged in monkey and mosquito cell lines infects human induced pluripotent stem cells (hiPSCs)-derived cortical neural progenitor cells (hNPCs) with much higher efficiency compared to hiPSCs and hNPC-derived immature cortical neurons. Infected hNPCs produces infectious ZIKV particles. Importantly, ZIKV infection increases cell death and dysregulates cell cycle progression, resulted in attenuated hNPC growth. RNA-sequencing analysis of infected hNPCs further reveals transcriptional dysregulation, notably genes in cell cycle pathways. Our results identify human cortical neural precursors as a major and direct target of ZIKV infection and establish a tractable experimental model system to investigate the impact and mechanism of ZIKV on human brain development and a platform to screen therapeutic compounds.
Overall design The suspected link between Zika virus infection and microcephaly is an urgent global health concern. Tang et al. report that Zika virus directly infects human cortical precursor cells with high efficiency, resulting in stunted growth and transcriptional dysregulation.
Contributor(s) Yao B
Citation(s) 26952870
Submission date Feb 26, 2016
Last update date May 15, 2019
Contact name Bing Yao
Phone 4047271725
Organization name Emory University
Department Human Genetics
Lab Bing Yao Lab
Street address 615 Michael St. Rm 323
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (2)
GPL15520 Illumina MiSeq (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM2073121 Mock1-1
GSM2073122 Mock2-1
GSM2073123 ZIKV1-1
BioProject PRJNA313294
SRA SRP070895

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Supplementary file Size Download File type/resource
GSE78711_Table.S2A.reads_R1.xlsx 14.1 Kb (ftp)(http) XLSX
GSE78711_gene.exp.all.txt.gz 373.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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