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Series GSE78521 Query DataSets for GSE78521
Status Public on May 13, 2016
Title Transcriptomes of individual substantia nigra pars reticulata neurons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Certain neuron types fire spontaneously at high rates, an ability that is crucial for their function in brain circuits. The spontaneously active GABAergic neurons of the substantia nigra pars reticulata (SNr), a major output of the basal ganglia, provide tonic inhibition of downstream brain areas. A depolarizing "leak" current supports this firing pattern, but its molecular basis remains poorly understood. To understand how SNr neurons maintain tonic activity, we used single-cell RNA sequencing to determine the transcriptome of individual SNr neurons. We discovered that SNr neurons express the sodium leak current, NaLCN and that SNr neurons lacking NaLCN have impaired spontaneous firing.
Overall design RNA sequencing profiles from 87 GFP-positive GABAergic SNr neurons and 9 GFP-negative SNr cells were carried out. However only 80 samples that passed initial quality control and that were included in the data processing are represented in this record.
Contributor(s) Lutas A, Lahmann C, Soumillon M, Yellen G
Citation(s) 27177420
Submission date Feb 25, 2016
Last update date May 15, 2019
Contact name Andrew Lutas
Organization name Harvard Medical School
Department Neurobiology
Lab Yellen
Street address 200 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (80)
GSM2072079 Sample 1
GSM2072080 Sample 2
GSM2072081 Sample 3
BioProject PRJNA313204
SRA SRP070835

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Supplementary file Size Download File type/resource
GSE78521_genes.fpkm_table.txt.gz 2.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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