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| Status |
Public on Feb 22, 2017 |
| Title |
Next generation sequencing reveals increased glycolysis and biomass production rate in mammary gland tumors of mice chronically exposed to insulin analogue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
For up to 70 weeks we subcutaneuously injected two hundered p53R270HWAPCre mice to different insulin-like molecules (regular insulin, insulin glargine, insulin X10 (of AspB10), IGF1 or vehicle solution). Due to the mammary gland specific p53 mutation the p53R270HWAPCre mice will develop spontanously human like mammary gland tumors in about a year. We found that frequent injections to insulin like molecules decreased the mammary gland tumor latency time in this model. Next we mRNA seqeunced tumors to reveal the underlying mechanisms for the increased tumor progression. For the next generation experiment we isolated mRNA from 50 tumors (10 tumors of each stimulation group) and sequenced with the IonTorrent (40 mil reads, on average 100 bp reads)
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| Overall design |
RNA expression profiles of 50 mammary gland tumors were analyzed, 10 tumors per treatment group (chronic insulin, glargine, x10, IGF1 or vehicle exposure)
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| Contributor(s) |
ter Braak B, van de Water B, van der Laan JW, Siezen KE, Rao P, Voorhoeve C, Ruppin E |
| Citation(s) |
28173837 |
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| Submission date |
Feb 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Bas ter Braak |
| E-mail(s) |
basterbraak@gmail.com
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| Organization name |
Leiden University/LACDR
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| Department |
Toxicology
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| Street address |
Einsteinweg 55
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| City |
Leiden |
| State/province |
Zuid-Holland |
| ZIP/Postal code |
2333CC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (50)
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| Relations |
| BioProject |
PRJNA312786 |
| SRA |
SRP070651 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE78156_IGF1.bigwig |
457.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE78156_RPKM_table.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
| GSE78156_X10.bigwig |
453.2 Mb |
(ftp)(http) |
BIGWIG |
| GSE78156_glargine.bigwig |
457.1 Mb |
(ftp)(http) |
BIGWIG |
| GSE78156_insulin.bigwig |
436.4 Mb |
(ftp)(http) |
BIGWIG |
| GSE78156_untreated.bigwig |
462.1 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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