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Series GSE7806 Query DataSets for GSE7806
Status Public on Sep 01, 2007
Title The effect of erstressin and nostressin on cytokine-induced A172 human glioblastoma cells in culture.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The unfolded protein response (UPR), a signaling pathway triggered by endoplasmic reticulum (ER) stress, is induced by a range of environmental factors. Here we describe the identification and characterization of a synthetic small molecule, erstressin, which activates the UPR. Erstressin induced rapid phosphorylation of PERK and eIF2a and the alternative splicing of XBP-1, hallmark initiating events of the UPR. Further, erstressin activated the transcription of multiple genes involved in the UPR. Coincident with these effects, erstressin also downregulated the transcription of the inflammation-associated enzyme inducible nitric oxide synthase (iNOS) in cytokine-activated cells. A close analog of erstressin that failed to induce the UPR did not attenuate expression of iNOS, suggesting that both biological effects of erstressin were mediated by a common mechanism. Further, the structurally-distinct ER stressor thapsigargin also inhibited iNOS expression. Together these chemical genetic studies reveal an unanticipated anti-inflammatory role for the UPR.
Keywords: Time course
Overall design We utilized microarrays to understand the global effect of a novel compund, erstressin, on cytokine stimulated cells over time.
Contributor(s) Dozier SJ, Hassig CA, Massari ME
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Submission date May 15, 2007
Last update date Dec 06, 2018
Contact name Sara Dozier
Organization name Kalypsys, Inc.
Street address 10420 Wateridge Circle
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (16)
GSM189431 30min_erstressin_biological_rep_1
GSM189432 30min_erstressin_biological_rep_2
GSM189433 30min_nostressin_biological_rep_1
BioProject PRJNA100023

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Supplementary file Size Download File type/resource
GSE7806_RAW.tar 29.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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