GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE78045 Query DataSets for GSE78045
Status Public on Nov 22, 2016
Title p53 is a critical regulator of airway epithelial progenitor cell homeostasis.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary How progenitor cells regulate quiescence and differentiation is poorly understood. Here, we demonstrate that the tumor suppressor p53 regulates both proliferation and differentiation of club progenitor cells in the airway epithelium. We show that p53 loss decreases ciliated cell differentiation and increases the proliferative capacity of club progenitors, increasing epithelial cell density. p53 deficient progenitors generated a pseudostratified epithelium containing basal-like cells in vitro and contained an increased proportion of BASCs in vivo, suggesting that p53 suppresses multipotency during homeostasis. Conversely, an additional copy of p53 decreases proliferation and increases ciliated cell differentiation. Using single cell RNA-Seq, we expose heterogeneity within airway epithelial progenitor cells and found that cell cycle regulators, particularly p21, are altered following p53 loss. Together, these findings reveal an essential role for p53 in regulating progenitor cell behavior, which has broad implications in understanding both stem cell and cancer biology
Overall design Examination of progenitor cell behavior in p53 knock out and wild type
Contributor(s) Farin AM, Yeckes AR, Wang Y, Selvagio AS, Gangalapudi V, Kirsch DG, Tang J, Stripp BR
Citation(s) 27880895
Submission date Feb 17, 2016
Last update date Oct 11, 2021
Contact name AGCT Core
Organization name Cedars Sinai Medical Center
Department BMS
Street address 8687 Melrose Ave, PDC B230
City West Hollywood
State/province CA
ZIP/Postal code 90069
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (150)
GSM2065421 p53-KO-A10-1-C52_S68
GSM2065422 p53-KO-A1-1-C03_S1
GSM2065423 p53-KO-A12-1-C54_S61
BioProject PRJNA312408
SRA SRP070468

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78045_20150923_Stripp_C1_WT.all.tpm.txt.gz 1.2 Mb (ftp)(http) TXT
GSE78045_20150924_Stripp_C1_p53_KO_Mouse.all.tpm.txt.gz 1.2 Mb (ftp)(http) TXT
GSE78045_low_input_RNA_d30_fpkm.txt.gz 733.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap