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Series GSE77796 Query DataSets for GSE77796
Status Public on Dec 31, 2016
Title Deep Sequencing Leads to the Identification of Eukaryotic Translation Initiation Factor 5A as a Key Element in Rsv1-Mediated Lethal Systemic Hypersensitive Response to Soybean Mosaic Virus Infection in Soybean
Organism Glycine max
Experiment type Expression profiling by high throughput sequencing
Other
Non-coding RNA profiling by high throughput sequencing
Summary We reported a genome-wide investigation to gain insight into the molecular signaling pathway involved in SMV G7 provoking Rsv1-mediated LSHR based on sRNA-seq, degradome-seq and transcriptome-seq analysis.
 
Overall design mRNA, miRNAs and degradome profiles of Rsv1-genotype soybean (PI96983) after 14 days post-inoculation (dpi) by SMV-L (avirulent strain), SMV-G7 (virulent strain) and Mock (control) were generated by deep sequencing, pooled from three biological replicates (5 plants in each replicate) for each treatment, using Illumina Miseq.
 
Contributor(s) Chen H, Arsovski AA, Yu K, Wang A
Citation(s) 27019403
Submission date Feb 10, 2016
Last update date Sep 23, 2019
Contact name Hui Chen
E-mail(s) Hui.Chen@agr.gc.ca
Phone 001-519-953-6710
Organization name Agriculture and Agri-Food Canada
Street address 1391 Sandford St.
City London
State/province Ontario
ZIP/Postal code N5V 4T3
Country Canada
 
Platforms (1)
GPL21309 Illumina MiSeq (Glycine max)
Samples (9)
GSM2059583 Rsv1_Mock_RNA-seq
GSM2059584 Rsv1_SMV-L_RNA-seq
GSM2059585 Rsv1_SMV-G7_RNA-seq
Relations
BioProject PRJNA311571
SRA SRP069875

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77796_Rsv1_RNA-seq_Normalized_Abundance_Measurements.xls.gz 2.4 Mb (ftp)(http) XLS
GSE77796_Rsv1_degradome_Normalized_Abundance_Measurements.xls.gz 1.3 Mb (ftp)(http) XLS
GSE77796_Rsv1_sRNA-seq_Normalized_Abundance_Measurements.xls.gz 41.6 Kb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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