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Status |
Public on Dec 06, 2017 |
Title |
hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export II |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To define the in vivo targets of the human nuclear exosome, we performed stranded RNA-seq using polyA RNAs isolated from nuclei of HeLa cells. To compare the RNA levels in each sample in an unbiased fashion, we added spike-in controls to equal amount of total nuclear RNAs.
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Overall design |
PolyA RNAs isolated from nuclei of control, hRRP40 or hMTR4 siRNA treated HeLa cells were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000.
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Contributor(s) |
Wu X, Li M |
Citation(s) |
28801509 |
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Submission date |
Feb 05, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Hong Cheng |
E-mail(s) |
hcheng@sibcb.ac.cn
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Organization name |
Shanghai Institutes for Biological Sciences
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Department |
Shanghai Institute of Biochemistry and Cell Biology
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Lab |
StateKey Laboratory of Molecular Biology
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Street address |
320 YueYang Road
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City |
Shanghai |
ZIP/Postal code |
200031 |
Country |
China |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE77641 |
hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export |
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Relations |
BioProject |
PRJNA311070 |
SRA |
SRP069362 |