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Series GSE77639 Query DataSets for GSE77639
Status Public on Dec 06, 2017
Title hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export II
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To define the in vivo targets of the human nuclear exosome, we performed stranded RNA-seq using polyA RNAs isolated from nuclei of HeLa cells. To compare the RNA levels in each sample in an unbiased fashion, we added spike-in controls to equal amount of total nuclear RNAs.
 
Overall design PolyA RNAs isolated from nuclei of control, hRRP40 or hMTR4 siRNA treated HeLa cells were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000.
 
Contributor(s) Wu X, Li M
Citation(s) 28801509
Submission date Feb 05, 2016
Last update date May 15, 2019
Contact name Hong Cheng
E-mail(s) hcheng@sibcb.ac.cn
Organization name Shanghai Institutes for Biological Sciences
Department Shanghai Institute of Biochemistry and Cell Biology
Lab StateKey Laboratory of Molecular Biology
Street address 320 YueYang Road
City Shanghai
ZIP/Postal code 200031
Country China
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM2055552 Sample 1-NC-1
GSM2055553 Sample 2-NC-2
GSM2055554 Sample 3-NC-3
This SubSeries is part of SuperSeries:
GSE77641 hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export
Relations
BioProject PRJNA311070
SRA SRP069362

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77639_processed_data.txt.gz 620.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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