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Series GSE7757 Query DataSets for GSE7757
Status Public on May 31, 2007
Title Robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies.
For the first time, we present a study focused on analyzing the impact of different RNA preparation procedures on gene expression data for different subtypes of pediatric acute leukemias.
Keywords: comparison of various total RNA preparation procedures
 
Overall design We systematically assessed the impact of three different total RNA isolation procedures on variation in expression data: method A: lysis of mononuclear cells, followed by lysate homogenization and RNA extraction; method B: organic solvent based RNA isolation, and method C: organic solvent based RNA isolation followed by purification.
 
Contributor(s) Campo Dell'Orto M, Kohlmann A
Citation(s) 17587440
Submission date May 08, 2007
Last update date Mar 25, 2019
Contact name Andrea Zangrando
E-mail(s) andrea.zangrando@unipd.it
Organization name University of Padova
Department Woman and Child's Health
Lab Hemato-Oncology
Street address Via Giustiniani, 3
City Padova
ZIP/Postal code 35138
Country Italy
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (99)
GSM187698 PAD_00232_sample 1_preparation A
GSM187699 PAD_00240_sample 1_preparation B
GSM187700 PAD_00248_sample 1_preparation C
Relations
BioProject PRJNA99883

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7757_RAW.tar 1.1 Gb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file

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