|
| Status |
Public on Jun 07, 2007 |
| Title |
Identification of human miR-34a-responsive transcripts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to examine the consequences of human miR-34a induction on the transcriptome, HCT116 cells (a colon cancer cell line) were infected with a retrovirus that produces miR-34a. Gene expression profiles were then monitored using Affymetrix microarrays.
Affymetrix microarrays were used to examine the transcriptomes of HCT116 cells infected with an empty retroviral vector (pMSCV-PIG) or a retroviral vector that expresses human miR-34a.
Keywords: comparison of cells with or without enforced miR-34a expression
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| |
|
| Overall design |
RNA from retrovirally-infected cells was isolated and hybridized to Affymetrix microarrays.
|
| |
|
| Contributor(s) |
Chang T, Wentzel EA, Mendell JT |
| Citation(s) |
17540599 |
| Submission date |
May 07, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Joshua T Mendell |
| E-mail(s) |
jmendell@jhmi.edu
|
| Phone |
410-502-0428
|
| Fax |
410-502-1853
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Institute of Genetic Medicine
|
| Street address |
733 N. Broadway, BRB 471
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (4)
|
| GSM187631 |
HCT116 cells with enforced miR-34a expression, technical replicate 1 |
| GSM187632 |
HCT116 cells with enforced miR-34a expression, technical replicate 2 |
| GSM187633 |
HCT116 cells with empty vector, technical replicate 1 |
| GSM187634 |
HCT116 cells with empty vector, technical replicate 2 |
|
| Relations |
| BioProject |
PRJNA99877 |
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