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| Status |
Public on Nov 17, 2016 |
| Title |
Arnica montana stimulates extracellular matrix gene expression in human macrophages differentiated to wound-healing phenotype. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Arnica m. effects were associated with a purported anti-inflammatory and tissue healing actions after trauma, bruises, or tissue injuries, but its cellular and molecular mechanisms are largely unknown. Here Arnica m. dilutions were tested using an in vitro model of macrophages polarized towards a “wound-healing” phenotype. The monocyte-macrophage human THP-1 cell line was cultured and differentiated with phorbol-myristate acetate and Interleukin-4, then exposed for 24 h to Arnica m. centesimal (c) dilutions 2c, 3c, 5c,9c, 15c or Control. None of these treatments affected cell viability. A total of 20 genes were differentially expressed comparing cells treated with Arnica m. 2c with those treated with Control only. Of these, 7 genes were up-regulated and 13 were down-regulated. Functional gene enrichment analysis showed that the most significantly upregulated function concerned 4 genes with a conserved site of EGF-like region (p<0.001) and three genes of proteinaceous extracellular matrix, including heparin sulphate proteoglycan 2 (HSPG2), fibrillin 2 (FBN2), and fibronectin (FN1) (p <0.01). Protein assay in supernatants confirmed a statistically significant increase of fibronectin production in Arnica m. 2c treated cells (p<0.05). Pooled extracts of cells treated with increasing dilutions of Arnica m. (3c, 5c, 15c) showed up-regulation of the same group of genes although with lower effect size. The down-regulated transcripts derive from mitochondrial genes coding for some components of electron transport chain. These findings provide new insights into the action of Arnica m. in tissue healing and repair, identifying increased fibronectin production by macrophages as a major therapeutic target.
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| Overall design |
Expression analysis of differentiated THP-1 cell line exposed at Arnica m. centesimal (c) dilution 2c, plus control non-exposed line both in 5 replicates.
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| Contributor(s) |
Marzotto M, Bonafini C, Olioso D, Bettinetti L, Di Leva F, Galbiati E, Bellavite P |
| Citation(s) |
27832158 |
| Submission date |
Jan 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Alberto Ferrarini |
| E-mail(s) |
alberto.ferrarini@univr.it
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| Phone |
+39-045-802-7058
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| Organization name |
University of Verona
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| Department |
Scientific and Technological Department
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| Lab |
Plant Functional Genomics Centre
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| Street address |
Strada le Grazie, 15
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| City |
Verona |
| State/province |
Veneto |
| ZIP/Postal code |
37134 |
| Country |
Italy |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (10)
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| GSM2051125 |
THP-1 cells untreated control_4 |
| GSM2051126 |
THP-1 cells untreated control_5 |
| GSM2051127 |
THP-1 cells exposed at Arnica m. centesimal dilution 2c_1 |
| GSM2051128 |
THP-1 cells exposed at Arnica m. centesimal dilution 2c_2 |
| GSM2051129 |
THP-1 cells exposed at Arnica m. centesimal dilution 2c_3 |
| GSM2051130 |
THP-1 cells exposed at Arnica m. centesimal dilution 2c_4 |
| GSM2051131 |
THP-1 cells exposed at Arnica m. centesimal dilution 2c_5 |
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| Relations |
| BioProject |
PRJNA310196 |
| SRA |
SRP069060 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE77382_expression_matrix.txt.gz |
963.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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