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Series GSE77382 Query DataSets for GSE77382
Status Public on Nov 17, 2016
Title Arnica montana stimulates extracellular matrix gene expression in human macrophages differentiated to wound-healing phenotype.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Arnica m. effects were associated with a purported anti-inflammatory and tissue healing actions after trauma, bruises, or tissue injuries, but its cellular and molecular mechanisms are largely unknown. Here Arnica m. dilutions were tested using an in vitro model of macrophages polarized towards a “wound-healing” phenotype. The monocyte-macrophage human THP-1 cell line was cultured and differentiated with phorbol-myristate acetate and Interleukin-4, then exposed for 24 h to Arnica m. centesimal (c) dilutions 2c, 3c, 5c,9c, 15c or Control. None of these treatments affected cell viability. A total of 20 genes were differentially expressed comparing cells treated with Arnica m. 2c with those treated with Control only. Of these, 7 genes were up-regulated and 13 were down-regulated. Functional gene enrichment analysis showed that the most significantly upregulated function concerned 4 genes with a conserved site of EGF-like region (p<0.001) and three genes of proteinaceous extracellular matrix, including heparin sulphate proteoglycan 2 (HSPG2), fibrillin 2 (FBN2), and fibronectin (FN1) (p <0.01). Protein assay in supernatants confirmed a statistically significant increase of fibronectin production in Arnica m. 2c treated cells (p<0.05). Pooled extracts of cells treated with increasing dilutions of Arnica m. (3c, 5c, 15c) showed up-regulation of the same group of genes although with lower effect size. The down-regulated transcripts derive from mitochondrial genes coding for some components of electron transport chain. These findings provide new insights into the action of Arnica m. in tissue healing and repair, identifying increased fibronectin production by macrophages as a major therapeutic target.
Overall design Expression analysis of differentiated THP-1 cell line exposed at Arnica m. centesimal (c) dilution 2c, plus control non-exposed line both in 5 replicates.
Contributor(s) Marzotto M, Bonafini C, Olioso D, Bettinetti L, Di Leva F, Galbiati E, Bellavite P
Citation(s) 27832158
Submission date Jan 29, 2016
Last update date May 15, 2019
Contact name Alberto Ferrarini
Phone +39-045-802-7058
Organization name University of Verona
Department Scientific and Technological Department
Lab Plant Functional Genomics Centre
Street address Strada le Grazie, 15
City Verona
State/province Veneto
ZIP/Postal code 37134
Country Italy
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM2051122 THP-1 cells untreated control_1
GSM2051123 THP-1 cells untreated control_2
GSM2051124 THP-1 cells untreated control_3
BioProject PRJNA310196
SRA SRP069060

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Supplementary file Size Download File type/resource
GSE77382_expression_matrix.txt.gz 963.9 Kb (ftp)(http) TXT
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Raw data are available in SRA

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