GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE77356 Query DataSets for GSE77356
Status Public on Jul 11, 2016
Title Different promoter affinities account for specificity in MYC-dependent gene regulation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Enhanced expression of the MYC transcription factor is observed in the majority of tumors. Two seemingly conflicting models have been proposed for its function: one proposes that MYC enhances expression of all genes, while the other model suggests gene-specific regulation. Here, we have explored the hypothesis that specific gene expression profiles arise since promoters differ in affinity for MYC and high-affinity promoters are fully occupied by physiological levels of MYC. We determined cellular MYC levels and used RNA- and ChIP-sequencing to correlate promoter occupancy with gene expression at different concentrations of MYC. Mathematical modeling showed that binding affinities for interactions of MYC with DNA and with core promoter- bound factors, such as WDR5, are sufficient to explain promoter occupancies observed in vivo. Importantly, promoter affinity stratifies different biological processes that are regulated by MYC, explaining why tumor-specific MYC levels induce specific gene expression programs and alter defined biological properties of cells.
Overall design Gene expression profiling at different MYC levels in a human tumor cell line and ChIP-Sequencing of MYC in these cells.
Contributor(s) Lorenzin F, Benary U, Baluapuri A, Walz S, Jung LA, von Eyß B, Kisker C, Wolf J, Eilers M, Wolf E
Citation(s) 27460974
Submission date Jan 28, 2016
Last update date May 15, 2019
Contact name Martin Eilers
Organization name University of Wuerzburg
Department Chair for Biochemistry and Molecular Biology
Lab Martin Eilers
Street address Am Hubland
City Wuerzburg
ZIP/Postal code 97074
Country Germany
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (22)
GSM2050623 RNAseq_depletion_siCTR_rep1
GSM2050624 RNAseq_depletion_siMYC_rep1
GSM2050625 RNAseq_depletion_siCTR_rep2
BioProject PRJNA310128
SRA SRP069027

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77356_RAW.tar 767.2 Mb (http)(custom) TAR (of WIG)
GSE77356_README.txt 949 b (ftp)(http) TXT
GSE77356_RNAseq_MYC_depletion_count_matrix.txt.gz 454.7 Kb (ftp)(http) TXT
GSE77356_RNAseq_MYC_depletion_logFC.txt.gz 638.6 Kb (ftp)(http) TXT
GSE77356_RNAseq_titration_count_matrix.txt.gz 569.7 Kb (ftp)(http) TXT
GSE77356_RNAseq_titration_logFC.txt.gz 1.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap