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Series GSE77352 Query DataSets for GSE77352
Status Public on Feb 11, 2017
Title A RUNX2-mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The goal is to identify transcripts that are regulated by RUNX2 in SAOS2 cells.
Overall design SAOS2 cells were transduced with lentiviruses expressing shLuc, shRUNX2_3 and shRUNX2_4 for 4 days followed by 2ug/ul puromycin selection to remove untransduced cells. A no-virus control was included to monitor the transduction efficiency. These control cells died after 2 days of puromycin selection. The lentivirally transduced cells were more than 80% survived, which indicates a high transduction efficiency. After four more days in puromycin, SAOS2 cells were harvested and subjected to total RNA extraction. 1ug total RNA were used to perform RNA-seq.
Contributor(s) Huang J
Citation(s) 26925584
Submission date Jan 28, 2016
Last update date May 15, 2019
Contact name Jing Huang
Organization name National Cancer Institute
Lab Cancer Biology and Genetics
Street address 37 Convent Dr. 37/3140
City Bethesda
State/province MD
ZIP/Postal code MD 20892
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (9)
GSM2050561 shLuc, repeat1
GSM2050562 shRUNX2_3_repeat1
GSM2050563 shRUNX2_4_repeat1
BioProject PRJNA310114
SRA SRP069018

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77352_RAW.tar 12.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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