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Series GSE77328 Query DataSets for GSE77328
Status Public on Oct 18, 2016
Title OmoMYC blunts promoter invasion by oncogenic MYC to inhibit gene expression characteristic of MYC-dependent tumors
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary MYC genes have both essential roles during normal development and exert oncogenic functions during tumorigenesis. Expression of a dominant-negative allele of MYC, termed OmoMYC, can induce rapid tumor regression in mouse models with little toxicity for normal tissues. How OmoMYC discriminates between physiological and oncogenic functions of MYC is unclear. We have solved the crystal structure of OmoMYC and show that it forms a stable homodimer and as such recognizes DNA in the same manner as the MYC/MAX heterodimer. OmoMYC attenuates both MYC-dependent activation and repression by competing with MYC/MAX for binding to chromatin, effectively lowering MYC/MAX occupancy at its cognate binding sites. OmoMYC causes the largest decreases in promoter occupancy and changes in expression on genes that are invaded by oncogenic MYC levels. A signature of OmoMYC-regulated genes defines subgroups with high MYC levels in multiple tumor entities and identifies novel targets for the eradication of MYC-driven tumors.
Overall design Gene expression changes and DNA binding of MYC in a cancer cell line expressing OmoMYC.
Contributor(s) Jung LA, Gebhardt A, Kölmel W, Ade CP, Walz S, Kuper J, von Eyß B, Letschert S, Redel C, d'Artista L, Biankin A, Zender L, Sauer M, Wolf E, Evan G, Kisker C, Eilers M
Citation(s) 27748763
Submission date Jan 28, 2016
Last update date May 15, 2019
Contact name Martin Eilers
Organization name University of Wuerzburg
Department Chair for Biochemistry and Molecular Biology
Lab Martin Eilers
Street address Am Hubland
City Wuerzburg
ZIP/Postal code 97074
Country Germany
Platforms (3)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (44)
GSM2049314 U2OS_ChIPseq_input
GSM2049315 U2OS_ChIPseq_OmoMYCwt_+Dox_HA
GSM2049316 U2OS_ChIPseq_vector_+Dox_HA
BioProject PRJNA310063
SRA SRP068991

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77328_KPC_RNAseq_logFC.txt.gz 950.1 Kb (ftp)(http) TXT
GSE77328_KPC_shRNA_screen_logFC.txt.gz 2.4 Kb (ftp)(http) TXT
GSE77328_RAW.tar 1.2 Gb (http)(custom) TAR (of WIG)
GSE77328_RNAseq_README.txt 2.9 Kb (ftp)(http) TXT
GSE77328_U2OS_RNAseq_logFC.txt.gz 2.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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