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Series GSE77319 Query DataSets for GSE77319
Status Public on May 09, 2016
Title Comparison of Bach2-tdRFP high expression and low expression NP-specific IgG1 light zone GC B cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Despite the importance of memory B cells for protection from recurrent infection, how these cells are selected during germinal center (GC) reactions remains unclear. We show here that light zone (LZ) GC B cells with lower affinity BCRs express a less CD40 signature and relatively high levels of Bach2, being prone to enter the memory B cell pool. We also find that Bach2 contributes to memory B cell generation in a Blimp-1-independent manner and that its higher expression confers on LZ GC cells a more advantage for entering the memory B cell compartment. Thus, our data support an instructive model in which weak T cell help keep Bach2 expression relatively high, thereby being predisposed to enter the memory pool.
Overall design mRNA expression profiles of Bach2-tdRFP low and high expression NP-specific IgG1 light zone GC B cells were generated by deep sequencing using Illumina HiSeq 1500.
Contributor(s) Kurosaki T, Shinnakasu R
Citation(s) 27158841
Submission date Jan 27, 2016
Last update date May 15, 2019
Phone +81-6-6879-4458
Organization name Osaka University Immunology Frontier Research Center
Lab Laboratory of Lymphocyte Differentiation
Street address 3-1 Yamada-oka
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (6)
GSM2049190 Bach2-tdRFP low 1
GSM2049191 Bach2-tdRFP low 2
GSM2049192 Bach2-tdRFP low 3
BioProject PRJNA310018
SRA SRP068978

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77319_RAW.tar 4.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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