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Series GSE77290 Query DataSets for GSE77290
Status Public on Jan 28, 2016
Title A NOVEL EPIGENETIC SILENCING PATHWAY INVOLVING the HIGHLY CONSERVED 5'-3' EXORIBONUCLEASE Dhp1/Rat1/Xrn2 in SCHIZOSACCHAROMYCES POMBE [CRAC-seq]
Organism Schizosaccharomyces pombe
Experiment type Other
Summary Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes.
In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including RNA interference (RNAi) and a 3’-5’ exoribonuclease complex exosome.
Here, we report a new RNA-processing pathway that contributes to epigenetic gene silencing and assembly of heterochromatin mediated by 5’-3’ exoribonuclease Dhp1/Xrn2.
Dhp1 mutation causes defective gene silencing both at peri-centromeric regions and at the silent mating type locus.
Intriguingly, mutation of either one of two well-characterized Dhp1-interacting proteins, the Din1 pyrophosphohydrolase or the Rhn1 transcription termination factor, does not show silencing defects at the main heterochromatic regions.
Dhp1 is essential in the sequential steps of establishing silencing in a manner independent of both RNAi and the exosome.
Genomic and genetic analysis suggest that Dhp1 is involved in post-transcriptional silencing of repetitive regions through its catalytic activity. Our study is the first investigation into an unexpected role of Dhp1/Rat1/Xrn2 in chromatin-based silencing. These results elucidate how various RNA-processing pathways, acting together or independently, contribute to epigenetic regulation of the eukaryotic genome.
 
Overall design CRAC-seq
 
Contributor(s) Tucker J, Ohle C, Schermann G, Bendrin K, Zhang W, Fischer T, Zhang K
Citation(s) 26889830
Submission date Jan 27, 2016
Last update date May 15, 2019
Contact name Tamas Fischer
E-mail(s) tamas.fischer@bzh.uni-heidelberg.de
Phone +49 6221 544728
Organization name Biochemie Zentrum Heidelebrg
Department BZH
Lab AG Fischer
Street address Im Neuenheimer Feld 328
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (5)
GSM2048199 Rpb2-HTP_WT_rep1
GSM2048200 Rpb2-HTP_WT_rep2
GSM2048201 Rpb2-HTP_Dhp1-1_rep1
This SubSeries is part of SuperSeries:
GSE77291 A NOVEL EPIGENETIC SILENCING PATHWAY INVOLVING the HIGHLY CONSERVED 5'-3' EXORIBONUCLEASE Dhp1/Rat1/Xrn2 in SCHIZOSACCHAROMYCES POMBE
Relations
BioProject PRJNA309977
SRA SRP068958

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77290_RAW.tar 164.1 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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