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Series GSE77122 Query DataSets for GSE77122
Status Public on Feb 10, 2017
Title Involvement of β-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Understanding the molecular defense mechanism of macrophages and identifying their effector molecules against malarial parasites may provide important clues for the discovery of new therapies. To analyze the immunological responses of malarial parasite-induced macrophages, we used DNA microarray technology to examine the gene profile of differentiated macrophages phagocytizing Plasmodium falciparum-parasitized erythrocytes (iRBCs). The transcriptional gene profile of macrophages in response to iRBCs represented 168 down-regulated genes, which were mainly involved in the cellular immune response, and 216 upregulated genes, which were involved in cellular proteolysis, growth, and adhesion. Importantly, the specific upregulation of β-defensin 130 (DEFB130) in these macrophages suggested a possible role for DEFB130 in malarial parasite elimination. Strikingly, differentiated macrophages phagocytizing iRBCs exhibited an increase in intracellular DEFB130 levels and DEFB130 appeared to accumulate at the site of iRBC engulfment. Furthermore, DEFB130 synthetic peptide exhibited a modest toxic effect on P. falciparum in vitro and P. yoelii in vivo, unlike scrambled DEFB130 peptide, which showed no antiplasmodial activity. Our data broaden our knowledge of the immunological response of macrophages to iRBCs and shed light on a new target for therapeutic intervention.
Overall design Human monocyte-derived macrophages were co-cultured with plasmodium falciparum-infected erythrocytes (iRBCs), saponin-treated iRBCs, or non-infected erythrocytes (RBCs).
Contributor(s) Takano R, Terkawi MA
Citation(s) 28181499
Submission date Jan 22, 2016
Last update date Nov 27, 2018
Contact name Ryo Takano
Phone +81-155-49-5645
Organization name Obihiro University of Agriculture and Veterinary Medicine
Department National Research Center for Protozoan Diseases
Lab Research Unit for Global Infection Control
Street address Inada-cho
City Obihiro
State/province Hokkaido
ZIP/Postal code 080-8555
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (8)
GSM2044537 iRBCs_rep1
GSM2044538 iRBCs_rep2
GSM2044539 iRBCs_rep3
BioProject PRJNA309501

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77122_RAW.tar 29.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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