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Series GSE77018 Query DataSets for GSE77018
Status Public on Oct 25, 2016
Title Antigen specific human conventional T cells (CD154+) or regulatory T cells (CD137+) directed agains A. fumigatus or Birch pollen lysate isolated from Birch pollen allergic patients or healthy controls.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Foxp3+ regulatory T cells (Treg) play a central role for tolerance against self and innocuous environmental antigens. However, the role of antigen-specificity for Treg-mediated tolerance is only incompletely understood. Here we show by direct ex vivo characterization of human CD4+ T cells, that the response against innocuous airborne antigens, such as plant pollen or fungal spores, is dominated by memory-like antigen-specific Treg. Surprisingly, breakdown of tolerance in atopic donors was not accompanied by a quantitatively or qualitatively altered Treg response, but instead correlated with a striking dichotomy of Treg versus Th2 target specificity. Allergenic proteins, are selectively targeted by Th2 cells, but not Treg. Thus human Treg specific for airborne antigens maintain tolerance at mucosal sites and the failure to generate specific Treg against a subgroup of antigens provides a window of opportunity for allergy development.
Overall design PBMCs from sex and age matched birch pollen allergic patients and healthy controls, were stimulated (7h) with airborne fungal (A. fumigatus) or birch pollen antigen (birch) and sorted into antigen specific conventional and regulatory T cells according to their expression of CD154+ and CD137+ on CD4+ T cells, respectively. Number of samples per group in parentheses: Healthy controls stimulated with A. fumigatus (n=5), allergic patients stimulated with A. fumigatus (n=6), healthy controls stimulated with birch (n=6), allergic patients stimulated with birch (n=4).
Contributor(s) Bacher P, Gerstmayer B, Rüberg S, Knauel M, Kollet J, Scheffold A, Heinrich F
Citation(s) 27773482
Submission date Jan 20, 2016
Last update date Jan 09, 2018
Contact name Jutta Kollet
Organization name Miltenyibiotec GmbH
Department Bioinformatics
Street address Friedrich-Ebert-Str. 68
City Bergisch Gladbach
ZIP/Postal code 51429
Country Germany
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (42)
GSM2042548 H3_Asp_Tcon
GSM2042549 H4_Asp_Tcon
GSM2042550 H5_Asp_Tcon
BioProject PRJNA309483

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Supplementary file Size Download File type/resource
GSE77018_RAW.tar 516.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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