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| Status |
Public on Mar 30, 2016 |
| Title |
ChIP-seq analysis of E2f4, H3K4me2 and H3K27ac in the mouse livers |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Arid1a is the subunit of SWI/SNF complex, which was reported to guide SWI/SNF to DNA. Here, we found that loss of Arid1a in the liver results in improved liver regeneration after partial hepatectomy.Genome-wide analysis showed that after hepatectomy,loss of Arid1a reduces the recruitment and activity of E2F4 on target promoters, resulting in expression programs that favor regeneration during injury. Correspondingly, these promoters showed increased H3k4me2 and H3k27ac marks, indicating de-repression of these E2f target genes.
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| Overall design |
The post partial hepatectomy mice liver cells were fixed and isolated, analysis of genomic occupancy of E2f4,H3K4me2,H3K27ac in hepatocytes from Arid1a WT and Arid1a liver specific KO mice by ChIP-seq.
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| Contributor(s) |
Sun X, Xing C, Kanchwala M, Zhu H |
| Citation(s) |
27044474 |
| Submission date |
Jan 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Xuxu Sun |
| Organization name |
shanghai jiao tong university
|
| Department |
school of medicine
|
| Street address |
227 south chongqing road
|
| City |
shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE76935 |
Suppression of SWI/SNF component Arid1a promotes mammalian regeneration through chromatin remodeling |
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| Relations |
| BioProject |
PRJNA308951 |
| SRA |
SRP068518 |
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