GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE76850 Query DataSets for GSE76850
Status Public on May 01, 2017
Title Acetyl-CoA metabolism by ACSS2 regulates neuronal histone acetylation and long-term memory [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Metabolic production of acetyl-CoA has been linked to histone acetylation and gene regulation, however the mechanisms are largely unknown. We show that the metabolic enzyme acetyl-CoA synthetase 2 (ACSS2) is a critical and directchum regulator of histone acetylation in neurons and of long-term mammalian memory. We observe increased nuclear ACSS2 in differentiating neurons in vitro. Genome-wide, ACSS2 binding corresponds with increased histone acetylation and gene expression of key neuronal genes. These data indicate that ACSS2 functions as a chromatin-bound co-activator to increase local concentrations of acetyl-CoA and to locally promote histone acetylation for transcription of neuron-specific genes. Remarkably, in vivo attenuation of hippocampal ACSS2 expression in adult mice impairs long-term spatial memory, a cognitive process reliant on histone acetylation. ACSS2 reduction in hippocampus also leads to a defect in upregulation of key neuronal genes involved in memory. These results reveal a unique connection between cellular metabolism and neural plasticity, and establish a link between generation of acetyl-CoA and neuronal chromatin regulation.
Overall design Genome-wide examination of histone H3 and H4 acetylation, as well as ACSS2 binding, in undifferentiated CAD cells and differentiated CAD neurons; background adjusted by H3 ChIP or Input.
Contributor(s) Mews P, Drake AM, Luczak V, Abel T, Berger SL
Citation(s) 28562591
Submission date Jan 13, 2016
Last update date May 15, 2019
Contact name Shelley L. Berger
Organization name University of Pennsylvania
Department Cell and Developmental Biology
Lab Berger
Street address 3400 CIVIC CENTER BLVD
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (14)
GSM2039009 H3K9ac_ChIPseq_undiff
GSM2039010 H4K5ac_ChIPseq_undiff
GSM2039011 H4K12ac_ChIPseq_undiff
This SubSeries is part of SuperSeries:
GSE76854 Acetyl-CoA metabolism by ACSS2 regulates neuronal histone acetylation and long-term memory
BioProject PRJNA308764
SRA SRP068434

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 168.0 Mb (ftp)(http) BW 126.2 Mb (ftp)(http) BW 132.2 Mb (ftp)(http) BW 159.2 Mb (ftp)(http) BW 106.6 Mb (ftp)(http) BW 129.8 Mb (ftp)(http) BW 170.8 Mb (ftp)(http) BW 122.6 Mb (ftp)(http) BW 142.6 Mb (ftp)(http) BW 159.0 Mb (ftp)(http) BW 102.4 Mb (ftp)(http) BW 134.0 Mb (ftp)(http) BW 153.1 Mb (ftp)(http) BW 84.7 Mb (ftp)(http) BW 129.2 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap