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Series GSE76805 Query DataSets for GSE76805
Status Public on Jan 13, 2016
Title Activity-induced Nr4a1 regulates spine density and distribution pattern of excitatory synapses in pyramidal neurons
Organism Mus musculus
Experiment type Expression profiling by array
Summary Excitatory synapses occur mainly on dendritic spines, and spine density is usually correlated with the strength of excitatory synaptic transmission. We report that Nr4a1, an activity-inducible gene encoding a nuclear receptor, regulates the density and distribution of dendritic spines in CA1 pyramidal neurons. Nr4a1 overexpression resulted in elimination of the majority of spines; however, postsynaptic densities were preserved on dendritic shafts, and the strength of excitatory synaptic transmission was unaffected, showing that excitatory synapses can be dissociated from spines. mRNA expression profiling studies suggest that Nr4a1-mediated transcriptional regulation of the actin cytoskeleton contributes to this effect. Under conditions of chronically elevated activity, when Nr4a1 was induced, Nr4a1 knockdown increased the density of spines and PSDs specifically at the distal ends of dendrites. Thus, Nr4a1 is a key component of an activity-induced transcriptional program that regulates the density and distribution of spines and synapses.
Overall design After 10 days in culture, dissociated mouse hippocampal neurons in 6-well plates were infected with lentivirus expressing either Flag-Nr4a1 or GFP and incubated for 6 days to allow for transgene expression. Total RNA was then isolated using RNeasy Plus kit (QIAGEN). Samples passing an mRNA quality check proceeded to quantitative analysis on Agilent-026655 4x44 Mouse Microarrays.
Contributor(s) Kaminker JS, Chen Y, Sheng M
Citation(s) 24976215
Submission date Jan 12, 2016
Last update date Jan 22, 2020
Contact name Josh Kaminker
Organization name Genentech
Department Bioinformatics and Computational Biology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (8)
GSM2038220 Control.1
GSM2038221 Control.2
GSM2038222 Control.3
BioProject PRJNA308606

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Supplementary file Size Download File type/resource
GSE76805_RAW.tar 34.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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