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Series GSE76687 Query DataSets for GSE76687
Status Public on Sep 15, 2016
Title Resetting epigenetic memory by reprogramming of histone modifications in mammals
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Polycomb group proteins and the related histone modification H3K27me3 can maintain the silencing of key developmental regulators and provide cellular memory. However, how such epigenetic state is reprogrammed and inherited between generations is poorly understood. Using an ultra-sensitive approach STAR ChIP-seq, we investigated H3K27me3 across 14 developmental stages along mouse gametogenesis and early development. Interestingly, highly pervasive H3K27me3 was found in regions depleted of transcription and DNA methylation in oocytes. Unexpectedly, we observed extensive loss of promoter H3K27me3 at Hox and other developmental genes upon fertilization. This is accompanied by global erasure of sperm H3K27me3 but inheritance of distal H3K27me3 from oocytes. The resulting allele-specific H3K27me3 patterns persist to blastocysts before being converted to canonical forms in postimplantation embryos, where both the H3K4me3/H3K27me3 bivalent promoter marks are restored at developmental genes. Together, these data revealed widespread resetting of epigenetic memory and striking plasticity of epigenome during gametogenesis and early development.
Overall design Mouse preimplantation embryos were obtained from crosses of C57BL/6N and PWK. A newly developed ChIP-seq approach (STAR ChIP-seq) and RNA-seq were performed in these embryos at variou stages in preimplantation development.
Contributor(s) Zheng H, Huang B, Zhang B, Xie W
Citation(s) 27635762
Submission date Jan 08, 2016
Last update date May 15, 2019
Contact name Bingjie Zhang
Organization name Tsinghua University
Street address 30 Shuangqing Rd
City Beijing
State/province Beijing
ZIP/Postal code 100190
Country China
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (27)
GSM2041066 ChIP-Seq PWK sperm K27Me3
GSM2041067 ChIP-Seq growing oocyte 7days K27Me3
GSM2041068 ChIP-Seq growing oocyte 14days K27Me3
BioProject PRJNA309055
SRA SRP068549

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76687_2cell_K27me3_maternal.bed.gz 33.3 Mb (ftp)(http) BED
GSE76687_2cell_K27me3_paternal.bed.gz 35.7 Mb (ftp)(http) BED
GSE76687_2cell_k27me3.bed.gz 72.5 Mb (ftp)(http) BED
GSE76687_2cell_k27me3_broadpeak.bed.gz 170.2 Kb (ftp)(http) BED
GSE76687_2cell_k27me3_maternal_broadpeak.bed.gz 205.0 Kb (ftp)(http) BED
GSE76687_2cell_k27me3_paternal_broadpeak.bed.gz 230.6 Kb (ftp)(http) BED
GSE76687_E6.5_epiblast_K27me3_maternal.bed.gz 27.1 Mb (ftp)(http) BED
GSE76687_E6.5_epiblast_K27me3_paternal.bed.gz 25.0 Mb (ftp)(http) BED
GSE76687_E6.5_epiblast_k27me3.bed.gz 64.2 Mb (ftp)(http) BED
GSE76687_E6.5_epiblast_k27me3_broadpeak.bed.gz 91.8 Kb (ftp)(http) BED
GSE76687_E6.5_epiblast_k27me3_maternal_broadpeak.bed.gz 112.1 Kb (ftp)(http) BED
GSE76687_E6.5_epiblast_k27me3_paternal_broadpeak.bed.gz 136.1 Kb (ftp)(http) BED
GSE76687_ICM_K27me3_maternal.bed.gz 15.0 Mb (ftp)(http) BED
GSE76687_ICM_K27me3_paternal.bed.gz 14.3 Mb (ftp)(http) BED
GSE76687_ICM_k27me3.bed.gz 55.4 Mb (ftp)(http) BED
GSE76687_ICM_k27me3_broadpeak.bed.gz 134.1 Kb (ftp)(http) BED
GSE76687_ICM_k27me3_maternal_broadpeak.bed.gz 185.3 Kb (ftp)(http) BED
GSE76687_ICM_k27me3_paternal_broadpeak.bed.gz 73.7 Kb (ftp)(http) BED
GSE76687_MII_oocyte_k27me3.bed.gz 47.2 Mb (ftp)(http) BED
GSE76687_MII_oocyte_k27me3_broadpeak.bed.gz 183.2 Kb (ftp)(http) BED
GSE76687_PN5_zygote_K27me3_maternal.bed.gz 25.1 Mb (ftp)(http) BED
GSE76687_PN5_zygote_K27me3_paternal.bed.gz 19.5 Mb (ftp)(http) BED
GSE76687_PN5_zygote_k27me3.bed.gz 74.3 Mb (ftp)(http) BED
GSE76687_PN5_zygote_k27me3_broadpeak.bed.gz 143.1 Kb (ftp)(http) BED
GSE76687_PN5_zygote_k27me3_maternal_broadpeak.bed.gz 198.1 Kb (ftp)(http) BED
GSE76687_PN5_zygote_k27me3_paternal_broadpeak.bed.gz 16.6 Kb (ftp)(http) BED
GSE76687_RAW.tar 1.3 Gb (http)(custom) TAR (of BED)
GSE76687_early_2cell_alpha_amanitin_FPKM.txt.gz 159.5 Kb (ftp)(http) TXT
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