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Series GSE76663 Query DataSets for GSE76663
Status Public on Jul 07, 2017
Title miRNAs affected by antagomiR-17 treatment
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To understand the the effect of antagomir-17 treatment on human endothelial cells derived from human umbilical cord blood (UCB) CD34+ hematopoietic stem cells, we have employed mRNA sequencing. The antagomiR-17 used in this study was purchased from Dharmacon and cell transfection was performed using Lipofectamine RNAiMAx from Life Technologies. Scramble antagomiR from Ambion was used as control. Cells were transfected with antagomiR-17 or scrambled antagomiR for 48 hours. After 48 h, the cells were collected, RNA was isolated and RNA samples were shipped to Exiqon Services, Denmark for mRNA sequencing. All sequencing experiments (RNA integrity measurements, library preparation and next generation sequencing) were conducted at Exiqon Services, Denmark.
Overall design CD34+ endothelial cells differentiated from umbilical cord blood hematopoietic stem cells (CD34+) were treated with 50 nM antagomiR-17 (Dharmacon) or scrambled antagomiR (Ambion) using Lipofectamine RNAiMAx (Life Technologies) for 48 h. Three replicates were used for each condition (i.e. antagomiR-17 and scramble antagomiR conditions).
Contributor(s) Aday S
Citation(s) 28963481
Submission date Jan 08, 2016
Last update date May 15, 2019
Contact name Sezin Aday
Organization name University of Pennsylvania
Street address 210 South 33rd Street, Skirkanich Hall
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM2033117 AntagomiR17-transfected CD34+-ECs, Replicate 1
GSM2033118 AntagomiR17-transfected CD34+-ECs, Replicate 2
GSM2033119 AntagomiR17-transfected CD34+-ECs, Replicate 3
BioProject PRJNA308287
SRA SRP068229

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Supplementary file Size Download File type/resource
GSE76663_cds.diff.gz 493.9 Kb (ftp)(http) DIFF
GSE76663_cds_exp.diff.gz 2.2 Mb (ftp)(http) DIFF
GSE76663_gene_exp.diff.gz 1.9 Mb (ftp)(http) DIFF
GSE76663_isoform_exp.diff.gz 7.7 Mb (ftp)(http) DIFF
GSE76663_promoters.diff.gz 1.2 Mb (ftp)(http) DIFF
GSE76663_splicing.diff.gz 1.7 Mb (ftp)(http) DIFF
GSE76663_tss_group_exp.diff.gz 3.7 Mb (ftp)(http) DIFF
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