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Series GSE7624 Query DataSets for GSE7624
Status Public on Mar 30, 2008
Title Expression Profiles of Monozygotic Twin
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The expression level for 15 887 transcripts in lymphoblastoid cell lines from 19 monozygotic twin pairs (10 male, 9 female) were analysed for the effects of genotype and sex. On an average, the effect of twin pairs explained 31% of the variance in normalized gene expression levels, consistent with previous broad sense heritability estimates. The effect of sex on gene expression levels was most noticeable on the X chromosome, which contained 15 of the 20 significantly differentially expressed genes. A high concordance was observed between the sex difference test statistics and surveys of genes escaping X chromosome inactivation. Notably, several autosomal genes showed significant differences in gene expression between the sexes despite much of the cellular environment differences being effectively removed in the cell lines. A publicly available gene expression data set from the CEPH families was used to validate the results. The heritability of gene expression levels as estimated from the two data sets showed a highly significant positive correlation, particularly when both estimates were close to one and thus had the smallest standard error. There was a large concordance between the genes significantly differentially expressed between the sexes in the two data sets. Analysis of the variability of probe binding intensities within a probe set indicated that results are robust to the possible presence of polymorphisms in the target sequences.
Keywords: Monozygotic twin pair Expression Profiles
 
Overall design Genome-wide gene expression in lymphoblastoid cell lines was determined using microarrays derived from 15 monozygotic (MZ) twin pairs (10 male, 9 female). 10 twin pair are discordant, 4 are concordant for a disease phenotype and their are 5 controls which have no disease phenotype. This data set was analyzed to interrupt replicated effects of sex and genotype, not disease characteristics. Subsets of these samples have been analyzed separately for disease characteristics, (GSE7036 – 3 MZ twins discordant for bipolar disorder) and (GSE7486 - 5 discordant and 4 concordant MZ twin pairs with idiopathic absence epilepsies and 5 unaffected MZ twin pairs).
 
Contributor(s) Matigian NA, McRae AF, Vadlamudi L, Mulley JC, Mowry B, Martin NG, Berkovic SF, Hayward NK, Visscher PM
Citation(s) 17164263
Submission date Apr 26, 2007
Last update date Mar 25, 2019
Contact name Nicholas Matigian
E-mail(s) n.matigian@griffith.edu.au
Phone 61 7 38753660
Organization name National Adult Stem Cell Centre
Lab Systems Biology
Street address 170 Kessels Rd, Nathan
City Brisbane
State/province Queensland
ZIP/Postal code 4111
Country Australia
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (35)
GSM162903 Trasnsformed Lymphocytes Unaffected Twin Pair 3 (DCBD3.2)(TP10)
GSM181371 LCL Discordant twin pair 1 (DCE1.1) (TP11)
GSM181372 LCL Discordant twin pair 1 (DCE1.2) (TP12)
Relations
BioProject PRJNA100247

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7624_RAW.tar 175.0 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file

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