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Series GSE76208 Query DataSets for GSE76208
Status Public on Mar 01, 2016
Title Effect of human DAI expressed by an oncolytic vaccinia virus on the gene expression of tumor cells (HS294T) and monocytes (THP-1).
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human melanoma tumor cells (HS294T) and monocytes (THP-1) were infected with a double deleted (-VGF, -TK) oncolytic vaccinia virus expressing human DAI (DNA-dependent activator of interferon-regulatory factors). Total RNA was collected and gene expresson profiles were determined with Agilent microarray. An oncolytic vaccinia virus that does not express DAI was used to control the effect of DAI and uninfected cells (PBS treated) were used to control the effect of virus infection.
In oncolytic virotherapy the ability of the virus to activate the immune system against tumors is nowadays generally understood to be a key mechanism in full eradication of cancer and for long-term anti-tumor effects. We armed an oncolytic vaccinia virus with DAI to increase the immunogenicity and the vaccine potency of the virus. The aim of this study was to study if the expression of DAI by a replicating vaccinia virus would alter the gene expression profile of infected cells and to study what are the differentially expressed genes.
Overall design Three-condition experiment: vvdd-tdTomato-hDAI vs. vvdd-tdTomato vs. PBS treated cells. 2 cell lines: HS294T tumor cells and THP-1 monocytes. 3 biological replicates of virus infected cells per cell line and 2 uninfected replicates per cell line. HS294T and THP-1 cells were treated with vvdd-tdTomato-hDAI or vvdd-tdTomato control virus, or with PBS only to have an uninfected control. 16 hours after infection total RNA was extracted and whole genome gene pfofiles were analyzed and differentially expressed genes determined.
Contributor(s) Hirvinen M, Fortino V, Greco D, Cerullo V
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Submission date Dec 21, 2015
Last update date Apr 23, 2018
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platforms (1)
GPL16699 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version)
Samples (16)
GSM1976545 MOCK_THP1_1
GSM1976546 HDAI_THP1_2
GSM1976547 MOCK_HS294T_1
BioProject PRJNA306644

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_1_1.txt.gz 5.9 Mb (ftp)(http) TXT
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_1_2.txt.gz 5.9 Mb (ftp)(http) TXT
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_1_3.txt.gz 5.9 Mb (ftp)(http) TXT
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_1_4.txt.gz 5.9 Mb (ftp)(http) TXT
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_2_1.txt.gz 5.8 Mb (ftp)(http) TXT
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_2_2.txt.gz 5.9 Mb (ftp)(http) TXT
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_2_3.txt.gz 5.9 Mb (ftp)(http) TXT
GSE76208_US11263921_253949430778_S01_GE2_1105_Oct12_2_4.txt.gz 5.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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