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Series GSE76172 Query DataSets for GSE76172
Status Public on Apr 12, 2016
Title Treatment of bone marrow-derived macrophages with hFc-FNDC4 recombinant protein
Organism Mus musculus
Experiment type Expression profiling by array
Summary FNDC4 is a novel secreted factor sharing high homology with the exercise-associated myokine irisin (FNDC5). Here we report that Fndc4 is robustly upregulated in various mouse models of inflammation as well as in human inflammatory conditions. Specifically, subjects with inflammatory bowel disease show increased FNDC4 levels locally at inflamed sites of the intestine. Interestingly, administration of recombinant FNDC4 during colitis development in mice resulted in markedly reduced disease severity compared to mice injected with a control protein. Conversely, mice that lacked Fndc4 showed increased colitis severity. Analysis of binding of FNDC4 to different immune cell types revealed strong and specific binding to macrophages and monocytes. FNDC4 treatment of bone marrow-derived macrophages in vitro resulted in reduced phagocytosis, improved survival and reduced pro-inflammatory chemokine expression. Hence, treatment with FNDC4 resulted in a state of dampened macrophage activity, while enhancing their survival. Thus, we have characterized a novel factor with direct therapeutic potential in inflammatory bowel disease and possibly other inflammatory diseases.
Overall design Bone marrow-derived macrophages were grown in growth media (DMEM, 10% FCS, 1% PenStrep and 20% L9209-media), starved for 24h in 1% FCS-DMEM and then treated for 24h in 1%FCS-DMEM with 100nM hFc-FNDC4 or hFc control protein.
Contributor(s) Bosma M, Boström PA
Citation(s) 27066907
Submission date Dec 18, 2015
Last update date Apr 18, 2017
Contact name Estelle Proux-Wéra
Organization name Science for Life laboratory
Department Department of Biochemistry and Biophysics
Street address P-Box 1031
City Solna
ZIP/Postal code 171 21
Country Sweden
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (6)
GSM1975764 hFc treated replicate 1
GSM1975765 hFc treated replicate 2
GSM1975766 hFc treated replicate 3
BioProject PRJNA307080

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Supplementary file Size Download File type/resource
GSE76172_RAW.tar 27.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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