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Series GSE76143 Query DataSets for GSE76143
Status Public on Dec 20, 2015
Title Zscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Zinc finger and SCAN domain-containing 10 (Zscan10, also known as Zfp206) encodes a transcription factor that has been reported to be involved in the maintenance of pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Zscan10 using the Cre-loxP system and analyzed its function. We succeeded in establishing Zscan10-null ES cells and confirmed their pluripotency by the generation of chimeric embryos. Our results clearly indicate that Zscan10 is dispensable for the ability of self-renewal and differentiation in ES cells.
Overall design An inducible knockout ES cell line of Zscan10 with the Cre-loxP system was generated and gene expression was measured without Zscan10 deletion or constitutive knocked out cells. Each sample was prepared in triplicate.
Contributor(s) Yamane M, Fujii S, Ohtsuka S, Niwa H
Citation(s) 26592664
Submission date Dec 18, 2015
Last update date May 15, 2019
Contact name Hitoshi Niwa
Organization name RIKEN
Department Center for Developmental Biology
Lab Laboratory for Pluripotent Stem Cell Studies
Street address 2-2-3 Minatojima-minamimachi,Chuo-ku
City Kobe
State/province Hyogo
ZIP/Postal code 650-0047
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (6)
GSM1974993 Zscan10_f/f-Tx_rep1
GSM1974994 Zscan10_f/f-Tx_rep2
GSM1974995 Zscan10_f/f-Tx_rep3
BioProject PRJNA306423
SRA SRP067556

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76143_RAW.tar 2.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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