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| Status |
Public on Jun 05, 2007 |
| Title |
Atm-/-,mTerc-/-,p53-/- triple knock-out lymphoma vs normal mouse DNA (GPL2884) |
| Organism |
Mus musculus |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
Highly rearranged and mutated cancer genomes present major challenges in the identification of pathogenetic events driving the cancer process. Here, we engineered lymphoma-prone mice with chromosomal instability to assess the utility of mouse models in cancer gene discovery and the extent of cross-species overlap in cancer-associated copy number aberrations. Integrating with targeted re-sequencing, our comparative oncogenomic studies efficiently identified FBXW7 and PTEN as commonly deleted or mutated tumor suppressors in human T-cell acute lymphoblastic leukemia/lymphoma (T-ALL). More generally, the murine cancers acquire widespread recurrent clonal amplifications and deletions targeting loci syntenic to alterations present in not only human T-ALL but also diverse tumors of hematopoietic, mesenchymal and epithelial types. These results thus support the view that murine and human tumors experience common biological processes driven by orthologous genetic events as they evolve towards a malignant phenotype. The highly concordant nature of genomic events encourages the use of genome unstable murine cancer models in the discovery of biologically relevant driver events in human cancer.
Keywords: comparative genomic hybridization, genetic modification
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| Overall design |
22 lymphoma samples from Atm-/-, mTerc-/-, p53-/- triple knock-out mice were analyzed. DNA from healthy mice of the same line was used as reference. Each sample was hybridized with dye-swap replica.
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| Contributor(s) |
Feng B, Maser R, Wong K, Protopopov A, Lin E, Chin L, Depinho R |
| Citation(s) |
17515920 |
| |
| Submission date |
Apr 24, 2007 |
| Last update date |
Feb 06, 2013 |
| Contact name |
bin feng |
| Organization name |
dfci
|
| Street address |
44 binney street
|
| City |
boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL2884 |
Agilent-013490 Mouse Genome CGH Microarray 44A G4414A (Feature Number version) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA105525 |
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