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Series GSE75968 Query DataSets for GSE75968
Status Public on Jun 01, 2016
Title microRNA expression profile related to lymph node status in women with stage grade 1-2-3 endometrial cancer
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The aim of this study was to evaluate whether microRNA profiles of grade 1 – 2 or 3 endometrioid adenocarcinomas can be related to nodal status and used as a tool to adapt surgical staging in early-stage EC.
MicroRNA expression were assessed in twelve formalin-fixed paraffin-embedded (FFPE) EC primary tumors with positive lymph node and in 36 FFPE EC primary tumors with negative lymph node, matched for grade, stage and lymphovascular space involvement status.
 
Overall design Total RNA was extracted using the miRNeasy FFPE Kit (Qiagen, Courtaboeuf, France), according to the manufacturer’s instructions. The 36 specimens of negative LN status were pooled by 3 in accordance with the matching criteria. Microarray analysis was conducted on 48 distinct specimens: Twelve specimens with positive LN and 36 specimens with negative LN. Microarray hybridization on miRNA 4.0 chips (Affymetrix) was conducted at the genomic platform of the Institut Cochin, Paris.
 
Contributor(s) Canlorbe G, Méhats C, Jacques S
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Submission date Dec 14, 2015
Last update date May 02, 2017
Contact name Geoffroy - CANLORBE
E-mail(s) geoffroy.canlorbe@aphp.fr
Phone +33 1 56 01 60 19
Organization name Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), University Pierre and Marie Curie, Paris 6
Department Department of Obstetrics and Gynaecology
Lab -
Street address 4, rue de la Chine
City Paris
State/province -
ZIP/Postal code 75020
Country France
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (24)
GSM1971071 G1_IA_ENEG_GPOS_1
GSM1971072 G1_IB_ENEG_GPOS_1
GSM1971073 G1_IB_ENEG_GPOS_2
Relations
BioProject PRJNA305874

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75968_RAW.tar 18.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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