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Series GSE75951 Query DataSets for GSE75951
Status Public on Oct 11, 2016
Title Transcriptome of mouse preimplantation development [Xist KO]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goals of this study are to establish a dynamic roadmap of imprinted X chromosome inactivation and the role of Xist by elucidation of the transcriptome of Xist KO embryos during mouse preimplantation development.

Methods: mRNA profiles of the preimplantation embryos WT and KO for Xist were elucidated by RNA-seq at various stages. Trophoblasts isolated from blastocyst outgrowths were also included. The sequence reads for samples where gender could be determined and that passed quality filters were analyzed at the level of autosomes, X chromosome as well as single genes.

Results: Female embryos fail to silence the X chromosome at late preimplantation development. General autosomal gene expression is not affected in embryos lacking Xist.

Conclusions: Xist is crucial for iXCI. In preimplantation embryos, the main in vivo function of Xist is to regulate iXCI in females.
Overall design Global mRNA profiles of single preimplantation embryos were generated by deep sequencing.
Contributor(s) Ingolf B
Citation(s) 27642011
Submission date Dec 11, 2015
Last update date May 15, 2019
Contact name Ingolf Bach
Organization name University of Massachusetts Medical School
Department Molecular, Cell and Cancer Biology
Street address 364 Plantation St
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (141)
GSM1970642 WT male 4 cell, repeat 1 [Xist KO]
GSM1970643 WT male 4 cell, repeat 2 [Xist KO]
GSM1970644 WT male 4 cell, repeat 3 [Xist KO]
This SubSeries is part of SuperSeries:
GSE71442 Transcriptome of mouse preimplantation development
BioProject PRJNA305764
SRA SRP067288

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75951_htseq_counts.txt.gz 2.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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