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Series GSE75844 Query DataSets for GSE75844
Status Public on Mar 31, 2016
Title Transposon mutagenesis reveals fludarabine-resistance mechanisms in chronic lymphocytic leukemia
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose:To identify resistance mechanisms for the chemotherapeutic drug fludarabine in chronic lymphocytic leukemia (CLL), as innate and acquired resistance to fludarabine-based chemotherapy represents a major challenge for long-term disease control.
Methods: We employed piggyBac transposon-mediated mutagenesis, combined with next-generation sequencing, to identify genes that confer resistance to fludarabine in a human CLL cell line.
Results: RNA-seq profiling of fludarabine-resistant cells suggested deregulated MAPK signaling as involved in mediating drug resistance in CLL.
Overall design To address if the fludarabine-resistant HG3 cells were transcriptionally different at a global level compared to their parental cells, we performed RNA-sequencing of three pairs of HG3 pools
Contributor(s) Hellström M
Citation(s) 26957556
Submission date Dec 09, 2015
Last update date May 15, 2019
Contact name L. H.
Organization name Uppsala University
Street address Dag Hammarskjölds Väg 20
City Uppsala
ZIP/Postal code 751 85
Country Sweden
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (6)
GSM1968781 control sample #17 TU
GSM1968782 control sample #18 TU
GSM1968783 control sample #28 TU
BioProject PRJNA305520
SRA SRP067175

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75844_RAW.tar 820.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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