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| Status |
Public on Jan 14, 2016 |
| Title |
Medial prefrontal cortex transcriptome changes in germ-free mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We sequenced mRNA from 12 samples extracted from mouse prefrontal cortex tissue to generate the first prefrontal cortex-specific murine transcriptome for germ-free mice (GF), conventionally raised controls (CON) and germ-free mice that have been colonized with normal microbiota from postnatal day 21 (exGF).
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| Overall design |
Equal amounts of RNA from two to three animals were pooled to yield 4 samples per group (CON, GF, and exGF). Pairwise comparisons for CONvsGF, CONvsexGF, GFvsexGF were performed using DESeq2.
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| Contributor(s) |
Hoban AE, Stilling RM, Ryan F, Clarke G, Claesson M, Cryan JF, Dinan TG, Shanahan F |
| Citation(s) |
27045844 |
| |
| Submission date |
Dec 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Roman M Stilling |
| E-mail(s) |
roman.stilling@gmail.com
|
| Organization name |
University College Cork
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| Department |
Anatomy and Neuroscience
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| Lab |
Neurogastroenterology
|
| Street address |
Western Road
|
| City |
Cork |
| ZIP/Postal code |
0 |
| Country |
Ireland |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA305425 |
| SRA |
SRP067146 |
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