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Series GSE75787 Query DataSets for GSE75787
Status Public on Jun 30, 2016
Title Loss of Asxl1 Alters Self-Renewal and Cell Fate of Bone Marrow Stromal Cell
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary De novo ASXL1 mutations are found in patients with Bohring-Opitz syndrome, a disease with severe developmental defects and early childhood fatality. The underlying pathologic mechanisms remain largely unknown. Using Asxl1-targeted murine models,we found that Asxl1 global loss or conditional deletion in osteoblasts and their progenitors in mice leads to significant bone loss and markedly decreased numbers of marrow mesenchymal stem/progenitor cells (MSPCs) compared with wild-type (WT) littermates. Asxl1-/- MSPCs displayed impaired self-renewal and skewed differentiation-away from osteoblasts and favoring adipocytes. RNA-seq analysis reveals the altered expression of genes involved in cell proliferation, skeletal development and morphogenesis. Furthermore, gene set enrichment analysis showed a decreased gene expression of stem cell self-renewal signature,suggesting the role of Asxl1 in regulating the stemness of MSPCs. Importantly, introducing Asxl1 normalized NANOG and OCT4 expression and restored the self-renewal capacity of Asxl1-/- MSPCs. Our study unveils a pivotal role of ASXL1 in maintenance of MSPC functions and skeletal development.
 
Overall design Examination of mRNA profiles in wild type and Asxl1-/- MSPCs by deep sequencing
 
Contributor(s) Zhang P, Xing C, Wang QF, Yang FC
Citation(s) 27237378
Submission date Dec 08, 2015
Last update date May 15, 2019
Contact name Caiying Zhu
E-mail(s) zhucaiying@ihcams.ac.cn
Phone 13622053408
Organization name Institute of Hematology & Blood Diseases Hospital
Street address NO. 288 Nanjing Road, Heping District, Tianjin
City Tianjin
ZIP/Postal code 300020
Country China
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM1968342 WT532
GSM1968343 WT535
GSM1968344 KO511
This SubSeries is part of SuperSeries:
GSE75788 Loss of Asxl1 Alters Mesenchymal Stem Cell Fate through H3K4me3
Relations
BioProject PRJNA305430
SRA SRP067150

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75787_ASXL1_cufflinks_GEP.txt.gz 932.7 Kb (ftp)(http) TXT
GSE75787_ASXL1_cufflinks_matrix_log10.txt.gz 593.1 Kb (ftp)(http) TXT
GSE75787_RAW.tar 3.0 Mb (http)(custom) TAR (of FPKM_TRACKING)
GSE75787_gene_exp.diff.txt.gz 760.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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