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Series GSE75786 Query DataSets for GSE75786
Status Public on Dec 08, 2018
Title Loss of Asxl1 Alters Mesenchymal Stem Cell Fate through H3K4me3 [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary De novo ASXL1 mutations are found in patients with Bohring-Opitz syndrome (BOS), a disease with severe developmental defects and early childhood fatality. The underlying pathologic mechanisms remain largely unknown. Using Asxl1-targeted murine models, we found that Asxl1 global loss or conditional deletion in osteoblasts and their progenitors in mice leads to significant bone loss and markedly decreased numbers of marrow mesenchymal stem/progenitor cells (MSPCs) compared with wild-type (WT) littermates. Asxl1 null MSPCs display impaired self-renewal and skewed differentiation from osteoblasts towards adipocytes. ChIP-seq data identified that ASXL1 and H3K4me3 co-occupy the promoter regions of genes critical for MSPC self-renewal. Loss of Asxl1 diminished the genome enrichment of H3K4me3. Combined analysis of RNA-seq and ChIP-seq data revealed that Asxl1 loss in MSPCs altered the expression of ASXL1/H3K4me3 target genes controlling self-renewal/lineage commitment. Our study unveil a pivotal role of ASXL1 in H3K4me3-associated bone homeostasis
Overall design Identification of ASXL1 binding profile in WT MSPCs and examination of 3 different histone modifications in WT and Asxl1-/- MSPCs.
Contributor(s) Zhang P, Xing C, Wang Q, Yang F
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Submission date Dec 08, 2015
Last update date May 15, 2019
Contact name Caiying Zhu
Phone 13622053408
Organization name Institute of Hematology & Blood Diseases Hospital
Street address NO. 288 Nanjing Road, Heping District, Tianjin
City Tianjin
ZIP/Postal code 300020
Country China
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM1968334 ASXL1_WT_ChIPSeq
GSM1968335 H3K4me2_WT_ChIPSeq
GSM1968336 H3K4me2_KO_ChIPSeq
This SubSeries is part of SuperSeries:
GSE75788 Loss of Asxl1 Alters Mesenchymal Stem Cell Fate through H3K4me3
BioProject PRJNA305431
SRA SRP067149

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75786_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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