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| Status |
Public on Dec 09, 2015 |
| Title |
C3A Cells: Exposure vs Control [one-channel arrays] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiles comparing FCCP, CCCP, Dinoterb, Benzoapyrene, TCP, Medium control, 0,1% DMSO in C3A cells
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| Overall design |
C3A cells were exposed for 2 hours to substances, 6 replicate wells per concentration were pooled. Total RNA was extracted using RNeasy Mini Kit (QUIAGEN, Product 74104, Hombrechtikon, Switzerland). Quality of extracted RNA was checked via the Nanodrop 1000 spectraphotomoeter V3.7, as well as running the samples on a 1.5% agarose gel. cDNA was generated and labeled with Cy3 or Cy5-CTP using the low RNA input linear amplification kit (Perkin-Elmer). Total RNA was reverse transcribed to cDNA using RNasin® Plus RNAse Inhibitor (N2611), M-MLV Reverse transcriptase (RNase H Minus, Point Mutant) (M3682), 5x Buffer (M3682) and random primer (C1181) from Promega AG (product number in brackets) (Promega AG, Dübendorf, Switzerland). For Microarray analysis Agilent SurePrint G3 Human Gene Expression Microarrays (8x60K) were used. Each treatment consisted of two biological replicates, the control consisted of three biological replicates.
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| Contributor(s) |
Legradi J, Hawliczek-Ignarski A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 08, 2015 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Jessica Legradi |
| E-mail(s) |
jessica.legradi@vu.nl
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| Organization name |
VU Amsterdam
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| Department |
IVM
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| Street address |
De Boelelaan 1087
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| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE75784 |
Mode of toxic action assignment of chemicals |
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| Relations |
| BioProject |
PRJNA305352 |
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