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Series GSE75783 Query DataSets for GSE75783
Status Public on Dec 09, 2015
Title C3A Cells: Exposure vs Control [one-channel arrays]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Gene expression profiles comparing FCCP, CCCP, Dinoterb, Benzoapyrene, TCP, Medium control, 0,1% DMSO in C3A cells
Overall design C3A cells were exposed for 2 hours to substances, 6 replicate wells per concentration were pooled. Total RNA was extracted using RNeasy Mini Kit (QUIAGEN, Product 74104, Hombrechtikon, Switzerland). Quality of extracted RNA was checked via the Nanodrop 1000 spectraphotomoeter V3.7, as well as running the samples on a 1.5% agarose gel. cDNA was generated and labeled with Cy3 or Cy5-CTP using the low RNA input linear amplification kit (Perkin-Elmer). Total RNA was reverse transcribed to cDNA using RNasin® Plus RNAse Inhibitor (N2611), M-MLV Reverse transcriptase (RNase H Minus, Point Mutant) (M3682), 5x Buffer (M3682) and random primer (C1181) from Promega AG (product number in brackets) (Promega AG, Dübendorf, Switzerland). For Microarray analysis Agilent SurePrint G3 Human Gene Expression Microarrays (8x60K) were used. Each treatment consisted of two biological replicates, the control consisted of three biological replicates.
Contributor(s) Legradi J, Hawliczek-Ignarski A
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Submission date Dec 08, 2015
Last update date Nov 27, 2018
Contact name Jessica Legradi
Organization name VU Amsterdam
Department IVM
Street address De Boelelaan 1087
City Amsterdam
ZIP/Postal code 1081 HV
Country Netherlands
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (16)
GSM1967620 Benzoapyrene Replicate1
GSM1967621 Benzoapyrene Replicate2
GSM1967622 CCCP Replicate 1
This SubSeries is part of SuperSeries:
GSE75784 Mode of toxic action assignment of chemicals
BioProject PRJNA305352

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75783_RAW.tar 198.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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