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| Status |
Public on Oct 20, 2016 |
| Title |
Temporal transcriptome analysis of control and Zeb2 knockout mESC in pluripotency and in neural differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To capture the Zeb2-dependent transcriptional changes in early cell state/fate decisions we performed RNA-seq on Zeb2 control and Zeb2 knockout cells. We chose three stages, which correspond in control ESCs to the naive pluripotent state (d0; very low amounts of Zeb2 mRNA), multipotent progenitors (d4, low Zeb2 mRNA/protein) and early neural progenitors (d6, high Zeb2 mRNA/protein), respectively.
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| |
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| Overall design |
Three biological replicates of Zeb2 control (Ctrl) and Zeb2 knockout (KO) samples on day 0, day 4 and day 6 of neural differentiation were used in this study (18 samples in total)
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| |
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| Contributor(s) |
Dries R, Stryjewska A, Huylebroeck D |
| Citation(s) |
27739137 |
| |
| Submission date |
Dec 02, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruben Dries |
| E-mail(s) |
rubendries@gmail.com
|
| Organization name |
DFCI
|
| Department |
Computational Biology
|
| Lab |
Longwood Center
|
| Street address |
360 Longwood Ave
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE75618 |
Temporal transcriptome and methylome analysis of differentiating mouse embryonic stem cells deficient for Zeb2 |
|
| Relations |
| BioProject |
PRJNA304747 |
| SRA |
SRP066910 |
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