GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE7561 Query DataSets for GSE7561
Status Public on Nov 20, 2008
Title Expression data from IGF-I-stimulated MCF-7 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Substantial evidence implicates IGF-I signaling in the development and progression of breast cancer. To identify transcriptional targets of IGF action in breast cancer cells, we performed gene expression profiling (>22,000 RNA transcripts) of IGF-I-stimulated MCF-7 cells, a well characterized breast cancer cell line that is highly responsive to IGFs. We defined an IGF-I gene signature pattern of hundreds of genes either up-regulated or down-regulated at both 3 and 24 hrs in vitro. After removing genes considered generic to cell proliferation, the signature was examined in four different public profile datasets of clinical breast tumors (representing close to 1000 patients), as well as in profile datasets of experimental models for various oncogenic signaling pathways. Genes with early and sustained regulation by IGF-I were highly enriched for transcriptional targets of the estrogen, Ras, and PI3K/Akt/mTOR pathways. The IGF-I signature appeared activated in most estrogen receptor-negative (ER-) clinical breast tumors and in a substantial subset (~25%) of ER+ breast tumors. Patients with tumors showing activation of the IGF-I signature tended to have a shorter time to disease recurrence (including patients not receiving adjuvant therapy), both when considering all patients and the subset of ER+ patients. We found evidence for cross-talk and common transcriptional endpoints between the IGF-I and estrogen systems. Our results support the idea that the IGF-I pathway is one mechanism by which breast tumors may acquire hormone independence and a more aggressive phenotype.
Keywords: two group comparison
Overall design MCF-7L cells were routinely maintained in DMEM + 5% FBS. Cells were plated in 10cm dishes and then next day incubated in serum-free medium. After an overnight incubation, cells were stimulated in triplicate with or without IGF-I (8nM or 50ng/ml) for 3 or 24hrs. Cells were then lysed and total RNA isolated using Qiagen midi-prep kit according to the manufacturer’s instructions. RNA extraction and hybridization was then carried out.
Contributor(s) Creighton C, Lee A
Citation(s) 18757322
Submission date Apr 20, 2007
Last update date Dec 06, 2018
Contact name Chad Creighton
Organization name Baylor College of Medicine
Department Biostatistics, Ducan Cancer Center
Street address One Baylor Plaza, Mail Stop: BCM305
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (11)
GSM183421 SFM control cells (3hr), biological rep1
GSM183422 SFM control cells (3hr), biological rep2
GSM183423 SFM control cells (24hr), biological rep1
BioProject PRJNA100357

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7561_RAW.tar 35.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap