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Series GSE75525 Query DataSets for GSE75525
Status Public on Dec 01, 2019
Title Epigenetic profiling of mouse bone marrow derived mast cells (BMMCs) [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Allergy is one of the least understood immunological response partly due to the diversity of allergens and the complexity of immune response against them. In allergic responses, mast cells are key players with their ability to rapidly degranulate and also generate de novo lipids and transcripts. Although the molecular details of degranulation in mast cell were studied in allergic inflammation, the transcriptional response involving chromatin remodeling, enhancer dynamics and long non-coding RNA (lncRNA) expression are largely unexplored. Here, using mouse bone marrow derived mast cells (BMMCs), we characterized the steady state, immunoglobulin E (IgE) and antigen (Ag) mediated epigenetic changes in mast cell chromatin and described the enhancer, super-enhancer and mRNA, lncRNA catalogue in these cells.
Overall design BMMCs were generated from mouse bone marrow cells with IL-3 and SCF. Cells were left untreated or sensitized overnight with DNP-IgE (0.5 ug/ml) and treated with DNP-BSA (50 ng/ml) for 2h. H3K4me1, H3K4me3 and H3K27Ac CHIP-seq was performed.
Contributor(s) Cildir G, Zhang J
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Submission date Nov 30, 2015
Last update date Dec 01, 2019
Contact name Jingxian Zhang
Organization name Institute of Molecular and Cell Biology (IMCB), A-Star, Singapore
Department IMCB
Street address 61 Biopolis Drive Proteos
City Singapore
State/province Singpaore
ZIP/Postal code 138673
Country Singapore
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (20)
GSM1958529 ChIP-Seq input for H3K4me1, H3K4me3, H3K27Ac rep 1
GSM1958530 ChIP-Seq input for H3K4me1, H3K4me3, H3K27Ac rep 2
GSM1958531 ChIP-Seq of H3K27ac in BMMC with DNP-BSA (50 ng/ml) for 2h rep 1
This SubSeries is part of SuperSeries:
GSE111547 Landscape of mouse mast cell chromatin: a rich resource for identification of novel mediators and genetic drivers of allergic and inflammatory diseases
BioProject PRJNA304469
SRA SRP066820

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Supplementary file Size Download File type/resource
GSE75525_RAW.tar 5.8 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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