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Series GSE75494 Query DataSets for GSE75494
Status Public on Dec 08, 2016
Title Transcriptional profiling of human ovarian cancer cells treated with ribonucleases
Organism Homo sapiens
Experiment type Expression profiling by array
Summary PE5 is a nuclear-directed human ribonuclease variant that exhibits selective cytotoxicity against cancer cells. Onconase is a natural cytotoxic ribonuclease that has reached advanced clinical trials for the treatment of different types of cancer. Onconase also posses established antiviral effects. In the present work, we have used global gene expression microarrays to study the effects caused by PE5 and onconase, and therefore elucidate the mechanism by which these ribonucleases induce cytotoxicity. Transcriptional profiling of human ovarian cancer cells treated with PE5 or onconase were compared with that of control untreated cells. The study were carried out with the multidrug resistance cell line NCI/ADR-RES and its parental cell line OVCAR8.
Overall design Two loop-design microarray experiments were performed, one for NCI/ADR-RES cell line and one for OVCAR8 cell line. Each loop contained six hybridizations (samples A-F and G-L) and three experimental conditions (control, PE5 treatment, and onconase treatment). Each loop employed four independent biological replicates.
Contributor(s) Vert A, Castro J, Ribó M, Benito A, Vilanova M
Citation(s) 28035074, 29379303
Submission date Nov 30, 2015
Last update date Aug 01, 2019
Contact name Maria Vilanova Brugués
Organization name Universitat de Girona
Department Biologia
Lab Laboratori d'Enginyeria de Proteïnes
Street address Maria Aurèlia Capmany 40
City Girona
ZIP/Postal code 17071
Country Spain
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (12)
GSM1957036 Control (Cy5) PE5 (Cy3) - NCI/ADR-RES
GSM1957037 PE5 (Cy5) Control (Cy3) - NCI/ADR-RES
GSM1957038 Control (Cy5) Onconase (Cy3) - NCI/ADR-RES
BioProject PRJNA304421

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75494_ADR-RES_vs_Control_OVCAR8.txt.gz 31.5 Kb (ftp)(http) TXT
GSE75494_All_log_ratios.xlsx 444.6 Kb (ftp)(http) XLSX
GSE75494_All_log_ratios_README.pdf 78.6 Kb (ftp)(http) PDF
GSE75494_RAW.tar 77.5 Mb (http)(custom) TAR (of TXT)
GSE75494_README.pdf 78.2 Kb (ftp)(http) PDF
GSE75494_log_ratios.txt.gz 64.8 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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