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Series GSE75477 Query DataSets for GSE75477
Status Public on Mar 17, 2016
Title Genome-wide analysis of chronic inflammation induced gene expression in livers isolated from either wild type or ApoE-Cyp7a1 transgenic animals.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Analysis of whole genome expression changes in livers from wild type animals and animals with a liver specific transgenic over expression of Cyp7a1. Mice were given a chronic, repetitive administration of LPS for 7 days. Our prior analysis had indicated that inflammation suppresses Cyp7a1 and that this leads to accumulation of intermediates in the mevalonate biosynthesis pathway. Here, we hypothesized that over expression of Cyp7a1 would not affect the changes in transcriptional state due to chronic administration of LPS. We provide gene expression data which evaluates this question. Here we find that over expression of Cyp7a1 minimally alters the transcriptome of livers in an untreated state, and that it has small effects on the response to chronic LPS.
Overall design Total RNA isolated from livers of wild type and liver specific Cyp7a1 transgenic animals treated with or without recurrent, daily LPS injections (1.5mg/kg) for 7 days. There are two biological replicates per condition. Samples are a matrix of all conditions reported as FPKMs.
Contributor(s) Okin D, Medzhitov R
Citation(s) 26997483
Submission date Nov 30, 2015
Last update date May 15, 2019
Contact name Daniel Okin
Organization name Yale University
Department Department of Immunobiology
Lab TAC S660
Street address PO Box 208011
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM1955693 Wild type, untreated, rep 1
GSM1955694 Wild type, untreated, rep 2
GSM1955695 Wild type, LPS treated, rep 1
BioProject PRJNA304393
SRA SRP066776

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75477_DAO32_FPKMsummaryFile.txt.gz 580.8 Kb (ftp)(http) TXT
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