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Series GSE75423 Query DataSets for GSE75423
Status Public on Oct 12, 2016
Title mRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify the mRNAs which regulate the decidualization of ECSCs, we explored differentially expressed mRNAs between untreated ECSCs (n=4) and decidualized ECSCs (n=4) using gene expression microarray. The mRNA expression profiles in untreated ECSCs and decidualized ECSCs were obtained, then the mRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 58% of cases have good flags). We established the criteria for regulated genes: Z-score ≥2.0 and ratio ≥2.0-fold for up-regulated genes, and Z-score ≤–2.0 and ratio ≤0.5 for down-regulated genes.
 
Overall design The primary culture of untreated ECSCs and decidualized ECSCs were utilized. Decidualization of cultured ECSCs was induced by 12 days-culture with a combination of 0.5 mM of dibutyryl-cAMP and 100 nM of dienogest in the presence of 10% charcoal-stripped heat-inactivated FBS. Total RNA was extracted from these samples, and gene expression microarray analysis was performed. The mRNAs differentially expressed between untreated ECSCs and decidualized ECSCs were identified, and aberrantly expressed mRNA in ECSCs were defined as the decidualization related mRNA.
 
Contributor(s) Aoyagi Y, Nasu K
Citation(s) 27412773
Submission date Nov 25, 2015
Last update date Mar 02, 2018
Contact name yoko aoyagi
E-mail(s) yokoao@oita-u.ac.jp
Organization name oita university
Street address 1-1 Idaigaoka hamasamachi
City yufu city
ZIP/Postal code 879-5593
Country Japan
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (8)
GSM1954898 ECSCs_12d_rep1
GSM1954899 ECSCs_12d_rep2
GSM1954900 ECSCs_12d_rep3
This SubSeries is part of SuperSeries:
GSE75427 Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)
Relations
BioProject PRJNA304173

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75423_RAW.tar 72.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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