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Series GSE75422 Query DataSets for GSE75422
Status Public on Oct 12, 2016
Title miRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To identify the miRNAs which regulate the decidualization of ECSCs, we explored differentially expressed miRNAs between untreated ECSCs (n=4) and decidualized ECSCs (n=4) using miRNA microarray. The miRNA expression profiles in untreated ECSCs and decidualized ECSCs were obtained, then the miRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 17% of cases have good flags). We established the criteria for regulated genes: ratio ≥2.0-fold for up-regulated genes and ratio ≤0.5 for down-regulated genes.
 
Overall design The primary culture of untreated ECSCs and decidualized ECSCs were utilized. Decidualization of cultured ECSCs was induced by 12 days-culture with a combination of 0.5 mM of dibutyryl-cAMP and 100 nM of dienogest in the presence of 10% charcoal-stripped heat-inactivated FBS. Total RNA was extracted from these samples, and miRNA microarray analysis was performed. The miRNAs differentially expressed between untreated ECSCs and decidualized ECSCs were identified, and aberrantly expressed miRNA in ECSCs were defined as the decidualization related miRNA.
 
Contributor(s) Aoyagi Y, Nasu K
Citation(s) 27412773
Submission date Nov 25, 2015
Last update date Mar 02, 2018
Contact name yoko aoyagi
E-mail(s) yokoao@oita-u.ac.jp
Organization name oita university
Street address 1-1 Idaigaoka hamasamachi
City yufu city
ZIP/Postal code 879-5593
Country Japan
 
Platforms (1)
GPL18402 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (miRNA ID version)
Samples (8)
GSM1954890 ECSCs_12d_rep1
GSM1954891 ECSCs_12d_rep2
GSM1954892 ECSCs_12d_rep3
This SubSeries is part of SuperSeries:
GSE75427 Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)
Relations
BioProject PRJNA304174

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75422_RAW.tar 71.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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