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Series GSE75386 Query DataSets for GSE75386
Status Public on Aug 18, 2016
Title Single cell RNAseq of electrophysiologically characterized neurons of the hippocampus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Recent advances in single-cell RNAseq technologies are enabling new cell type classifications. For neurons, electrophysiological properties traditionally guide cell type classification but correlating RNAseq data with electrophysiological parameters has been difficult. Here we demonstrate RNAseq of electrophysiologically and synaptically characterized individual, patched neurons in the hippocampal CA1-region and subiculum, and relate the resulting transcriptome data to their electrical and synaptic properties. In this analysis, we explored the hypothesis that precise combinatorial interactions between matching cell-adhesion and signaling molecules shape synapse specificity. In analyzing interneurons and pyramidal neurons that are synaptically connected, we identified two independent, developmentally regulated networks of interacting genes encoding cell-adhesion, exocytosis and signal-transduction molecules. In this manner, our data allow postulating a presumed cell-adhesion and signaling code, which may explain neuronal connectivity at the molecular level. Our approach enables correlating electrophysiological with molecular properties of neurons, and suggests new avenues towards understanding synaptic specificity.
Overall design These data include 15 tissue samples (including 3 independent replicas in 5 developmental stages) as well as 93 single-cell samples (including CA1 cholecystokinin, parvalbumin, and pyramidal neurons as well as subiculum burst and regular firing pyramidal neurons).
Contributor(s) Földy C, Darmanis S, Aoto J, Malenka R, Quake SR, Südhof TC
Citation(s) 27531958
Submission date Nov 24, 2015
Last update date May 15, 2019
Contact name Spyros Darmanis
Phone 6506960861
Organization name Stanford University
Department Bioengineering
Lab Stephen Quake
Street address Clark Center, E300
City Stanford
State/province California
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (108)
GSM2026617 C100
GSM2026618 C101
GSM2026619 C102
BioProject PRJNA307566
SRA SRP068021

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Supplementary file Size Download File type/resource
GSE75386_Darmanis_hippocampus_processed_data_all.csv.gz 1.5 Mb (ftp)(http) CSV
GSE75386_RAW.tar 8.6 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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