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Series GSE75378 Query DataSets for GSE75378
Status Public on Nov 25, 2015
Title The effect of palmitate on gene expression profile in proliferating myoblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary High-fat diet or exposure to saturated fatty acids affects skeletal muscle growth and function. The aim of the present study was to investigate the effect of palmitate on the transcriptomic profile of mouse C2C12 myoblasts. Global gene expression was evaluated using whole mouse genome oligonucleotide microarrays, and the results were validated through real-time PCR. A total of 4047genes were identified as differentially expressed, including 3492 down-regulated and 555 up-regulated genes during 48-h-exposure to palmitate (0.1 mmol/l). Functional classification showed the involvement of PA-regulated genes in several processes which regulate cell growth. In conclusion, palmitate modifies the expression of genes associated with: i) myoblast responsiveness to hormones and growth factors, ii) cytokine and growth factor expression, and iii) regulation of cell-cell cell-matrix communication. Such alterations can affect myoblast growth and differentiation, however further studies in this field are required.
To whom the correspondence should be addressed: Dr K. Grzelkowska-Kowalczyk; e-mail:, tel/fax: (48 22) 847 24 52
Overall design After scanning of hybridized microarrays, quantitation of slide images was performed using Feature Extraction Software (Agilent) using default parameters and the raw data were exported to GeneSpring GX 12 (Agilent, Santa Clara, CA) and log2 transformed. For identification of genes significantly altered in cell compared with the control normal gene set. Total detected entities were filtered by flags (detected, non detected) and error (coefficient of variation: CV < 50.0 percent) to remove very low signal entities and to select reproducible signal values of entities among the replicated experiments, respectively. In statistical analysis, separated for experiment with myoblasts treated with palmitate acid was used t-test unpaired (p < 0.05) with multiple testing correction: Benjamini-Hochberg <0.05, all significant changes over fold change 1.6 were selected.
Contributor(s) Grabiec K, Majewska A, Wicik Z, Milewska M, Błaszczyk M, Grzelkowska-Kowalczyk K
Citation(s) 27114085
Submission date Nov 24, 2015
Last update date Jul 24, 2019
Contact name Zofia Wicik
Organization name Mossakowski Medical Research Center
Department Department of Human Epigenetics
Street address Pawinskiego 5
City Warsaw
State/province mazovia
ZIP/Postal code 02-106
Country Poland
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (4)
GSM1954220 myoblasts treated with palmitate (0.1 mmol/l)- rep1
GSM1954221 myoblasts treated with palmitate (0.1 mmol/l)- rep2
GSM1954222 myoblasts treated with palmitate (0.1 mmol/l)- rep3
BioProject PRJNA304026

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75378_RAW.tar 16.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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