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Series GSE75250 Query DataSets for GSE75250
Status Public on Nov 21, 2015
Title Genome-wide profilings of IFN-stimulated murine splenic B cells by ChIP-seq.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the IFN-induced dynamics in murine splenic B cells. Male C57BL/6 mice were injected subcutaneously with 10,000U IFNa and spleens were removed at designated time. B cells were negatively isolated using magnetic beads and profiled for the binding of PolII and Stat2 by ChIP-seq analysis.
Overall design Profilings by PolII ChIP-seq over the time course (0, 0.5, 1, 2, 4, 8, 24 and 48hrs, single replicate for each), Stat2 ChIP-seq (0 and 90min, biological duplicate for each).
Contributor(s) Mostafavi S, Yoshida H, Moodley D, Yang L, Mathis D, Benoist C
Citation(s) 26824662
Submission date Nov 20, 2015
Last update date May 15, 2019
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (17)
GSM1947436 pol2_0hr
GSM1947437 pol2_30min
GSM1947438 pol2_1hr
This SubSeries is part of SuperSeries:
GSE75306 ImmGen Cytokines: Interferons
BioProject PRJNA302909
SRA SRP066492

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Supplementary file Size Download File type/resource
GSE75250_RAW.tar 2.3 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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